beta-Carotene promotes the development of NNK-induced small airway-derived lung adenocarcinoma.
beta-Carotene promotes the development of NNK-induced small airway-derived lung adenocarcinoma.
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DOI：
10.1016/j.ejca.2008.10.035
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发表时间：
2009-05
影响因子：
8.4
通讯作者：
Schuller, Hildegard M.
中科院分区：
文献类型：
--
作者：
Al-Wadei, Hussein A. N.;Schuller, Hildegard M.
β -胡萝卜素在临床前研究中显示出癌症预防作用，而在临床试验中却增加了肺癌死亡率。我们已经证明β-胡萝卜素在体外刺激cAMP信号。在这里，我们验证了β -胡萝卜素通过cAMP信号传导促进体内肺腺癌（PAC）发展的假设。方法：用致癌物4-（甲基亚硝胺）-1-（3-吡啶基）-1-丁酮（NNK）和β-胡萝卜素对仓鼠进行PAC诱导，持续1.5年。记录肺肿瘤的发生率、多样性和大小，Western blots检测肿瘤细胞中CREB和ERK1/2的磷酸化水平。免疫分析法分析血细胞中的环AMP，高效液相色谱法分析血清和肺中的类维生素a。结果：β -胡萝卜素增加了肺肿瘤的多样性、肺肿瘤的大小、血细胞cAMP、血清和肺中类维生素a的水平，并诱导了肺肿瘤的p-CREB和p-ERK1/2。结论：我们的数据表明β -胡萝卜素通过增加cAMP信号通路促进PAC的发展。
Beta-carotene has shown cancer preventive effects in preclinical studies while increasing lung cancer mortality in clinical trials. We have shown that β-carotene stimulates cAMP signaling in vitro. Here, we have tested the hypothesis that beta-carotene promotes the development of pulmonary adenocarcinoma (PAC) in vivo via cAMP signaling. Methods: PAC was induced in hamsters with the carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) followed by β-carotene for 1.5 years. Incidence, multiplicity and size of lung tumors were recorded and phosphorylated CREB and ERK1/2 in tumour cells determined by Western blots. Cyclic AMP in blood cells was analysed by immunoassays, retinoids in serum and lungs by HPLC. Results: beta-carotene increased lung tumor multiplicity, lung tumour size, blood cell cAMP, serum and lung levels of retinoids and induced p-CREB and p-ERK1/2 in lung tumours. Conclusions:Our data suggest that beta-carotene promotes the development of PAC via increased cAMP signaling.
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影响因子：
6.4
作者：
Ezzati, M;Henley, SJ;Thun, MJ
通讯作者：
Thun, MJ
影响因子：
4.7
作者：
OREFFO, VIC;LIN, HW;WITSCHI, H
通讯作者：
WITSCHI, H
影响因子：
3.6
作者：
Schuller, HM;Porter, B;Riechert, A
通讯作者：
Riechert, A
DOI：
10.1046/j.1440-1681.2002.03625.x
发表时间：
2002-03-01
影响因子：
2.9
作者：
Dzimiri, N;Basco, C;Al-Halees, Z
通讯作者：
Al-Halees, Z
DOI：
10.1073/pnas.241500798
发表时间：
2001-11-20
影响因子：
11.1
作者：
Garber, ME;Troyanskaya, OG;Petersen, I
通讯作者：
Petersen, I