Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.
Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.
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DOI：
10.1111/imb.12038
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发表时间：
2013-10
影响因子：
2.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Yang N;Xie W;Jones CM;Bass C;Jiao X;Yang X;Liu B;Li R;Zhang Y
烟粉虱对包括新烟碱类在内的许多杀虫剂产生了高水平的抗药性，有强有力的证据表明，对某些化合物的抗药性是阶段特有的。为了研究烟粉虱对新烟碱类硫氧磷的抗性的分子基础，我们利用自制的烟粉虱基因芯片比较了烟粉虱抗新烟碱甲氧西林品系(TH-R)和敏感品系(TH-S)在卵期、若虫期和成虫期的基因表达。基因本体论和生物信息学分析表明，在所有生命阶段，许多差异表达的转录本编码参与外源生物代谢过程和/或代谢的酶。其中一些是候选抗性基因，包括细胞色素P450CYP6CM1，属于细胞色素P450S4家族的P450，以及属于Sigma类的谷胱甘肽S转移酶。最后，ABCG亚家族的几个ATP结合盒转运体在TH-R株成虫阶段高度过度表达，并可能通过主动外排在抗性中发挥作用。在这里，我们评估了共同的和阶段特定的基因表达特征，并确定了几个候选抗性基因，这些基因可能是烟粉虱对甲氧西林抗性的基础。
Bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific. To investigate the molecular basis of B. tabaci resistance to the neonicotinoid thiamethoxam we used a custom whitefly microarray to compare gene expression in the egg, nymph and adult stages of a thiamethoxam-resistant strain (TH-R) with a susceptible strain (TH-S). Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics. Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class. Finally several ATP-binding cassette transporters of the ABCG subfamily were highly over-expressed in the adult stage of the TH-R strain and may play a role in resistance by active efflux. Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B. tabaci resistance to thiamethoxam.
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