Desulfo-glucosinolate sulfotransferases isolated from several Arabidopsis thaliana ecotypes differ in their sequence and enzyme kinetics.
Desulfo-glucosinolate sulfotransferases isolated from several Arabidopsis thaliana ecotypes differ in their sequence and enzyme kinetics.
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从几种拟南芥生态型中分离的脱硫芥子油苷磺基转移酶在序列和酶动力学方面存在差异
DOI：
10.1016/j.plaphy.2012.11.005
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Papenbrock
中科院分区：
文献类型：
--
作者：
Luczak;Forlani;Papenbrock
目的是研究不同拟南芥（Arabidopsis thaliana L.）嘿生态类型至少部分地由磺基转移酶（SOTs）（EC 2.8.2.-）的动力学性质形成催化G1核心结构生物合成的最后一步。本研究仅关注有助于GI生物合成的三种SOT中的一种。AtSOT 18蛋白的同源物的特征在于，这是受到早期发现的SOTs从生态型Col-0和C24不同的两个氨基酸（aa）和特定的酶活性。AtSOT 18酶活性和GI谱的差异是否存在相关性？SOT 18序列从八个拟南芥生态型具有高度多样性的G1模式不同的两个氨基酸在不同的位置在蛋白质序列。来自Col-0的SOT 18序列显示出与比对中最大数量的其他序列的最高相似性。一级序列的微小差异导致二级和三级结构的重要结构变化，这可能是对广泛底物的不同动力学活性的关键。所有的重组AtSOT 18蛋白显示出与吲哚Gl的低底物特异性，而对脂肪族底物的特异性不同。在各生态型中，动力学行为与主要的ds-Gl含量或C3/C4ds-Gl比率没有相关性。因此，ds-GlAtSOT 18蛋白不可能在形成拟南芥中的GI谱中起主要作用。
The goal was to investigate whether the diverse glucosinolate (Gl) profiles described for different Arabidopsis thaliana (L.) Heynh. ecotypes are at least partially shaped by the kinetic properties of sulfotransferases (SOTs) (EC 2.8.2.-) catalyzing the final step in Gl core structure biosynthesis. This study focuses on only one of the three SOTs that contribute to Gl biosynthesis. Homologues of AtSOT18 proteins were characterized, which was inspired by earlier findings on SOTs from ecotypes Col-0 and C24 differing in two amino acids (aa) and specific enzyme activities. Could there be a correlation of AtSOT18 enzyme activities and differences in Gl profiles between the ecotypes? SOT18 sequences from eight Arabidopsis ecotypes with highly diverse Gl patterns differed in two aa at various positions in the protein sequence. The SOT18 sequence from Col-0 showed the highest similarity to the largest number of other sequences in the alignment. The small differences in the primary sequence lead to important structural changes in secondary and tertiary structure that might be the key of different kinetic activities towards a broad range of substrates. All recombinant AtSOT18 proteins showed low substrate specificity with an indolic Gl, while the specificity for aliphatic substrates varied. There is no correlation in the kinetic behavior with the major ds-Gl contents or with the ratio of C3/C4ds-Gl in the respective ecotype. Therefore, is it unlikely that ds-Gl AtSOT18 proteins play a major role in shaping the Gl profile in Arabidopsis.
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