Accounting for blood attenuation in intravascular near-infrared fluorescence-ultrasound imaging using a fluorophore-coated guidewire.
Accounting for blood attenuation in intravascular near-infrared fluorescence-ultrasound imaging using a fluorophore-coated guidewire.
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DOI：
10.1117/1.jbo.28.4.046001
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发表时间：
2023-04
影响因子：
3.5
通讯作者：
中科院分区：
文献类型：
--
作者：
血管内近红外荧光（NIRF）成像旨在利用对血管疾病生物标志物具有特异性的荧光剂改善血管病理检查。该方法已被开发用于与解剖学模式（如血管内超声（IVUS））串联操作，并与病理生理对比补充解剖学读数，增强从混合检查中获得的信息。然而，血液中NIRF信号的衰减对NIRF的量化提出了挑战。我们提出了一种基于荧光团涂层导丝的NIRF血管内成像衰减校正新方法，该导丝用作荧光测量的参考，并在NIRF检查期间提供血液衰减的实时测量。我们使用3.2F nif - ivus导管在猪冠状动脉离体和幻象中检查该方法的性能。我们证明了与未经校正的信号相比有显著改善，高达4.5倍，并且在距离所采用的导管系统1mm的距离处获得的目标信号误差为。该方法提供了一种在体内条件下提高血管内NIRF成像准确性的潜在手段。
Intravascular near-infrared fluorescence (NIRF) imaging aims to improve the inspection of vascular pathology using fluorescent agents with specificity to vascular disease biomarkers. The method has been developed to operate in tandem with an anatomical modality, such as intravascular ultrasound (IVUS), and complements anatomical readings with pathophysiological contrast, enhancing the information obtained from the hybrid examination. However, attenuation of NIRF signals by blood challenges NIRF quantification. We propose a new method for attenuation correction in NIRF intravascular imaging based on a fluorophore-coated guidewire that is used as a reference for the fluorescence measurement and provides a real-time measurement of blood attenuation during the NIRF examination. We examine the performance of the method in a porcine coronary artery ex vivo and phantoms using a 3.2F NIRF-IVUS catheter. We demonstrate marked improvement over uncorrected signals of up to 4.5-fold and errors of for target signals acquired at distances up to 1 mm from the catheter system employed. The method offers a potential means of improving the accuracy of intravascular NIRF imaging under in vivo conditions.
影响因子：
3.5
作者：
Mallas G;Brooks DH;Rosenthal A;Nudelman RN;Mauskapf A;Jaffer FA;Ntziachristos V
通讯作者：
Ntziachristos V
影响因子：
2.8
作者：
Kellnberger S;Wissmeyer G;Albaghdadi M;Piao Z;Li W;Mauskapf A;Rauschendorfer P;Tearney GJ;Ntziachristos V;Jaffer FA
通讯作者：
Jaffer FA