Constitutive Expressor Of Pathogenesis-related Genes5 affects cell wall biogenesis and trichome development.
Constitutive Expressor Of Pathogenesis-related Genes5 affects cell wall biogenesis and trichome development.
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DOI：
10.1186/1471-2229-8-58
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发表时间：
2008-05-16
影响因子：
5.3
通讯作者：
Larkin, John C.
中科院分区：
文献类型：
--
作者：
Brininstool, Ginger;Kasili, Remmy;Simmons, L. Alice;Kirik, Viktor;Huelskamp, Martin;Larkin, John C.
拟南芥CPR5基因与抗病性、细胞增殖、细胞死亡和糖敏感有关，编码一种具有未知生物化学功能的膜蛋白。毛状体发育在cpr 5植物中也受到影响，其具有尺寸和分支数量减少的叶毛状体。在这里介绍的工作中，CPR5在毛状体发育中的作用进行了检查。cpr5突变体上的毛状体具有降低的双折射，表明cpr5和野生型毛状体之间的细胞壁结构的差异。与此一致，cpr 5植物的叶细胞壁含有显着较少的次晶纤维素，并改变了壁碳水化合物的组成。我们还发现，cpr5对毛状体大小和毛状体核DNA的内复制的影响是上位性的triptychon（try）突变或GLABRA3过表达的影响，表明这些毛状体发育调节剂依赖于CPR5功能对毛状体扩展和内复制的影响。我们的研究结果表明，CPR5不太可能是病原体反应途径或衰老的特定调节因子，而是在细胞壁生物发生或多个细胞信号传导或转录反应途径中起作用。
The Arabidopsis thaliana CONSTITUTIVE EXPRESSOR OF PATHOGENESIS-RELATED GENES5 (CPR5) gene has been previously implicated in disease resistance, cell proliferation, cell death, and sugar sensing, and encodes a putative membrane protein of unknown biochemical function. Trichome development is also affected in cpr5 plants, which have leaf trichomes that are reduced in size and branch number. In the work presented here, the role of CPR5 in trichome development was examined. Trichomes on cpr5 mutants had reduced birefringence, suggesting a difference in cell wall structure between cpr5 and wild-type trichomes. Consistent with this, leaf cell walls of cpr5 plants contained significantly less paracrystalline cellulose and had an altered wall carbohydrate composition. We also found that the effects of cpr5 on trichome size and endoreplication of trichome nuclear DNA were epistatic to the effects of mutations in triptychon (try) or overexpression of GLABRA3, indicating that these trichome developmental regulators are dependant on CPR5 function for their effects on trichome expansion and endoreplication. Our results suggest that CPR5 is unlikely to be a specific regulator of pathogen response pathways or senescence, but rather functions either in cell wall biogenesis or in multiple cell signaling or transcription response pathways.
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影响因子：
64.5
作者：
HULSKAMP, M;MISERA, S;JURGENS, G
通讯作者：
JURGENS, G
影响因子：
11.6
作者：
Schnittger, A;Weinl, C;Hülskamp, M
通讯作者：
Hülskamp, M
影响因子：
11.6
作者：
Bowling, SA;Clarke, JD;Dong, XN
通讯作者：
Dong, XN
影响因子：
11.6
作者：
Clarke, JD;Volko, SM;Dong, XN
通讯作者：
Dong, XN
影响因子：
9.2
作者：
Kirik, V;Bouyer, D;Hülskamp, M
通讯作者：
Hülskamp, M