Channel properties reveal differential expression of TARPed and TARPless AMPARs in stargazer neurons.
Channel properties reveal differential expression of TARPed and TARPless AMPARs in stargazer neurons.
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作者：
Dynamic regulation of calcium-permeable (CP-) AMPARs is important in normal synaptic transmission, plasticity and pathological changes. While the involvement of TARPs in trafficking of calcium-impermeable (CI-) AMPARs has been extensively studied, their role in surface expression and function of CP-AMPARs remains unclear. Here we examined AMPAR-mediated currents in cerebellar stellate cells from stargazer mice, lacking the prototypical TARP stargazin (γ-2). We found a marked increase in the contribution of CP-AMPARs to synaptic responses, indicating that, unlike CI-AMPARs, these can localize at synapses in the absence of γ-2. In contrast with CP-AMPARs in extrasynaptic regions, synaptic CP-AMPARs displayed an unexpectedly low channel conductance and strong block by intracellular spermine, suggesting they were ‘TARPless’. As proof of principle that TARP-association is not an absolute requirement for AMPAR clustering at synapses, mEPSCs mediated by TARPless AMPARs were readily detected in stargazer granule cells following knock down of their only other TARP, γ-7.
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影响因子：
4.8
作者：
Bedoukian, Matthew A.;Weeks, Autumn M.;Partin, Kathryn M.
通讯作者：
Partin, Kathryn M.
影响因子：
64.8
作者：
Chavez, Andres E.;Singer, Joshua H.;Diamond, Jeffrey S.
通讯作者：
Diamond, Jeffrey S.
DOI：
10.1523/jneurosci.5134-10.2011
发表时间：
2011-03-16
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Jackson AC;Nicoll RA
通讯作者：
Nicoll RA
影响因子：
2.9
作者：
Fukaya, M;Yamazaki, M;Watanabe, M
通讯作者：
Watanabe, M
影响因子：
5.3
作者：
Kato, Akihiko S.;Zhou, Wei;Bredt, David S.
通讯作者：
Bredt, David S.