Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
复制标题
单丹磺基戊烷作为多色活细胞成像的蓝色荧光脂滴标记物
作者：
Huei;Chia;Jin;W. Yang
脂滴是一种负责储存中性脂质的动态细胞器，与多种代谢综合征有关。在这里，我们报告了单十二烷基戊烷(MDH)作为LDS的高对比度蓝色荧光标记物。独特的光谱特性使MDH很容易与其他绿色和红色荧光报告相结合，进行多色荧光成像。MDH染色不会明显影响LD的运输，而且该染料具有极好的光稳定性。综上所述，MDH是利用荧光显微镜研究活细胞内动态LD调节的可靠工具。
Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.
影响因子：
56.9
作者：
Terskikh, A;Fradkov, A;Siebert, P
通讯作者：
Siebert, P