Low-level vagus nerve stimulation upregulates small conductance calcium-activated potassium channels in the stellate ganglion.
Low-level vagus nerve stimulation upregulates small conductance calcium-activated potassium channels in the stellate ganglion.
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DOI：
10.1016/j.hrthm.2013.01.029
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发表时间：
2013-06
期刊：
影响因子：
5.5
通讯作者：
Chen, Peng-Sheng
中科院分区：
文献类型：
--
作者：
Shen, Mark J.;Chang, Hao-Che;Park, Hyung-Wook;Akingba, A. George;Chang, Po-Cheng;Zhang, Zheng;Lin, Shien-Fong;Shen, Changyu;Chen, Lan S.;Chen, Zhenhui;Fishbein, Michael C.;Chiamvimonvat, Nipavan;Chen, Peng-Sheng
关键词：
小电导钙激活钾 (SK) 通道负责抑制神经放电的后超极化。测试低水平迷走神经刺激 (LL-VNS) 导致 LSG 中 SK2 蛋白上调的假设。六只狗（第 1 组）接受了为期 1 周的左颈迷走神经 LL-VNS 治疗。五只正常狗（第2组）用作对照。通过蛋白质印迹法检查SK2蛋白水平。 SK2与3-磷酸甘油醛脱氢酶(GAPDH)水平之间的比率用作任意单位(AU)。我们发现第 1 组 (0.124 ± 0.049 AU) 中的 SK2 表达高于第 2 组 (0.085 ± 0.031 AU，P < 0.05)。免疫染色显示，第1组的SK2抗体染色的神经结构密度（11,546 ± 7,271 μm2/mm2）也高于第2组（5,321 ± 3,164 μm2/mm2，P < 0.05）。第 1 组中对 TH 无免疫反应性的神经节细胞 (11.4 ± 2.3%) 明显多于第 2 组 (4.9 ± 0.7%；P < 0.05)。 TH 阴性神经节细胞大多呈胆碱乙酰转移酶 (ChAT) 染色阳性（第 1 组为 95.9 ± 2.8%，第 2 组为 86.1 ± 4.4%，P = 0.10）。免疫荧光共聚焦显微镜显示，与第2组相比，第1组细胞质中的SK2染色显着减少，但神经节细胞膜上的SK2染色增加。左侧LL-VNS导致SK2蛋白上调，细胞膜中SK2蛋白表达增加，以及LSG中TH阴性（主要是ChAT阳性）神经节细胞增加。这些变化可能是 LL-VNS 对行走犬的抗心律失常功效的基础。
Small conductance calcium activated potassium (SK) channels are responsible for afterhyperpolarization that suppresses nerve discharges. To test the hypotheses that low-level vagus nerve stimulation (LL-VNS) leads to the upregulation of SK2 proteins in the LSG. Six dogs (Group 1) underwent 1-wk LL-VNS of the left cervical vagus nerve. Five normal dogs (Group 2) were used as control. SK2 protein levels were examined by western blotting. The ratio between SK2 and glyceraldehydes-3-phosphate-dehydrogenase (GAPDH) levels was used as an arbitrary unit (AU). We found higher SK2 expression in Group 1 (0.124 ± 0.049 AU) than Group 2 (0.085 ± 0.031 AU, P < 0.05). Immunostaining showed that the density of nerve structures stained with SK2 antibody was also higher in Group 1 (11,546 ± 7,271 μm2/mm2) than in Group 2 (5,321 ± 3,164 μm2/mm2, P < 0.05). There were significantly more ganglion cells without immunoreactivity to TH in Group 1 (11.4 ± 2.3%) than Group 2 (4.9 ± 0.7%; P < 0.05). The TH-negative ganglion cells mostly stained positive for choline acetyltransferase (ChAT) (95.9 ± 2.8% in Group 1 and 86.1 ± 4.4% in Group 2, P = 0.10). Immunofluorescence confocal microscopy revealed a significant decrease in the SK2 staining in the cytosol but an increase in the SK2 staining on the membrane of the ganglion cells in Group 1 compared to Group 2. Left LL-VNS results in the upregulation of SK2 proteins, increased SK2 protein expression in the cell membrane and the increased TH-negative (mostly ChAT-positive) ganglion cells in the LSG. These changes may underlie the antiarrhythmic efficacy of LL-VNS in ambulatory dogs.
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影响因子：
20.1
作者：
Chua SK;Chang PC;Maruyama M;Turker I;Shinohara T;Shen MJ;Chen Z;Shen C;Rubart-von der Lohe M;Lopshire JC;Ogawa M;Weiss JN;Lin SF;Ai T;Chen PS
通讯作者：
Chen PS
影响因子：
4.8
作者：
Ren, YJ;Barnwell, LF;Anderson, AE
通讯作者：
Anderson, AE
影响因子：
20.1
作者：
Cao, JM;Chen, LS;Chen, PS
通讯作者：
Chen, PS
影响因子：
24
作者：
Ogawa, Masahiro;Zhou, Shengmei;Chen, Peng-Sheng
通讯作者：
Chen, Peng-Sheng
DOI：
10.1073/pnas.91.15.7109
发表时间：
1994-07-19
影响因子：
11.1
作者：
BANNER, LR;PATTERSON, PH
通讯作者：
PATTERSON, PH