Deficits of olfactory interneurons in polysialyltransferase‐ and NCAM‐deficient mice
Deficits of olfactory interneurons in polysialyltransferase‐ and NCAM‐deficient mice
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聚唾液酸转移酶â和NCAMâ缺陷小鼠的嗅觉中间神经元缺陷
DOI：
10.1002/dneu.22324
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发表时间：
2016
影响因子：
3
通讯作者：
Hildebrandt H
中科院分区：
文献类型：
--
作者：
Röckle I;Hildebrandt H
室管膜下前区（SVZ）的神经源性小生境持续产生神经母细胞，这些神经母细胞沿着吻侧迁移流（RMS）迁移到嗅球（OB），在那里它们分化成颗粒细胞和球周细胞。神经细胞粘附分子NCAM或其翻译后修饰聚唾液酸（polySia）的缺失会损害迁移，导致近端RMS中细胞积聚和OB体积减少。NCAM的多聚唾液酸化通过两种多聚唾液酸转移酶ST 8 SIA 2和ST 8 SIA 4实现，具有重叠的功能。在这里，我们使用Ncam 1和多聚唾液酸转移酶缺失的小鼠来分析polySia合成的部分或完全丧失或polySia和NCAM的联合丧失如何影响RMS和OB中的中间神经元组成。在Ncam 1敲除、St 8 sia 2、St 8 sia 4双敲除和St 8 sia 2、St 8 sia 4、Ncam 1三敲除小鼠以及St 8 sia 2 −/−但St 8 sia 4 −/−小鼠中，检测到大量钙视网膜蛋白（CR）阳性细胞分散在RMS周围。这些变化未通过OB颗粒或肾小球层中CR阳性细胞的减少反映出来。相反，在所有polySia-NCAM阴性小鼠中，钙结合蛋白阳性的肾小球周围中间神经元强烈减少，在St 8 sia 2 −/−和St 8 sia 4 −/−小鼠中轻微减弱，这些小鼠在RMS上没有异位CR阳性细胞沿着。与CR阳性OB中间神经元相比，钙结合蛋白的早期发育一代一致，这种表型在出生后第5天完全发育。总之，这些结果表明，钙结合蛋白阳性肾小球周围中间神经元的早期发育取决于NCAM上polySia的呈递，并且需要两种聚唾液酸转移酶的活性。© 2015威利期刊公司.开发神经生物学76：421-433，2016
The neurogenic niche of the anterior subventricular zone (SVZ) persistently generates neuroblasts, which migrate along the rostral migratory stream (RMS) into the olfactory bulb (OB), where they differentiate into granule and periglomerular cells. Loss of the neural cell adhesion molecule NCAM or its post‐translational modification polysialic acid (polySia) impairs migration causing accumulations of cells in the proximal RMS and decreased OB volume. Polysialylation of NCAM is implemented by two polysialyltransferases, ST8SIA2 and ST8SIA4, with overlapping functions. Here, we used mice withNcam1and polysialyltransferase deletions to analyze how partial or complete loss of polySia synthesis or a combined loss of polySia and NCAM affects the RMS and the interneuron composition in the OB. Numerous calretinin (CR)‐positive cells were detected dispersed around the RMS inNcam1knockout,St8sia2,St8sia4double‐knockout, andSt8sia2,St8sia4, Ncam1triple‐knockout mice, as well as inSt8sia2−/−but not inSt8sia4−/−mice. These changes were not reflected by reductions of CR‐positive cells in the granule or glomerular layer of the OB. Instead, calbindin‐positive periglomerular interneurons were strongly reduced in all polySia‐NCAM negative mice and slightly attenuated inSt8sia2−/−as well as in theSt8sia4−/−mice, which were devoid of ectopic CR‐positive cells along the RMS. Consistent with the early developmental generation of calbindin‐ as compared with CR‐positive OB interneurons, this phenotype was fully developed at postnatal day 5. Together, these results demonstrate that the early development of calbindin‐positive periglomerular interneurons depends on the presentation of polySia on NCAM and requires the activity of both polysialyltransferases. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 421–433, 2016
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影响因子：
4.4
作者：
Muehlenhoff, Martina;Rollenhagen, Manuela;Hildebrandt, Herbert
通讯作者：
Hildebrandt, Herbert
影响因子：
6.6
作者：
B. Turetsky;C. Hahn;Karin E. Borgmann-Winter;P. Moberg
通讯作者：
B. Turetsky;C. Hahn;Karin E. Borgmann-Winter;P. Moberg
影响因子：
4.8
作者：
Weinhold, B;Seidenfaden, R;Hildebrandt, H
通讯作者：
Hildebrandt, H
DOI：
10.1073/pnas.0406082102
发表时间：
2005-07-05
影响因子：
11.1
作者：
Yamaguchi, M;Mori, K
通讯作者：
Mori, K
影响因子：
5.3
作者：
Battista, Daniela;Rutishauser, Urs
通讯作者：
Rutishauser, Urs