Hydrophobic derivatization of N-linked glycans for increased ion abundance in electrospray ionization mass spectrometry.
Hydrophobic derivatization of N-linked glycans for increased ion abundance in electrospray ionization mass spectrometry.
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DOI：
10.1007/s13361-011-0140-x
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发表时间：
2011-08
影响因子：
3.2
通讯作者：
Muddiman, David C.
中科院分区：
文献类型：
--
作者：
Walker, S. Hunter;Lilley, Laura M.;Enamorado, Monica F.;Comins, Daniel L.;Muddiman, David C.
合成了一个中性疏水试剂库，用作衍生剂，以增加电喷雾电离质谱（ESI MS）中N-连接聚糖的离子丰度。聚糖通过形成腙衍生化，并显示可使聚糖标准品的离子丰度增加4倍以上。此外，数据显示，系统地向试剂中添加疏水表面积增加了聚糖离子丰度，这是一种可以在聚糖分析中进一步利用的特性。本研究的结果将指导未来的疏水性试剂的合成用于聚糖分析使用疏水性和理论非极性表面积计算之间的相关性，以促进开发最佳的标签聚糖衍生化。通过切割和衍生人血浆蛋白中的N-连接聚糖证明了该方法的兼容性和优势。标记聚糖的ESI-MS信号显着更丰富，并且带负电荷的唾液酸化聚糖的检测得到增强。
A library of neutral, hydrophobic reagents was synthesized for use as derivatizing agents in order to increase the ion abundance of N-linked glycans in electrospray ionization mass spectrometry (ESI MS). The glycans are derivatized via hydrazone formation and are shown to increase the ion abundance of a glycan standard more than 4-fold. Additionally, the data show that the systematic addition of hydrophobic surface area to the reagent increases the glycan ion abundance, a property that can be further exploited in the analysis of glycans. The results of this study will direct the future synthesis of hydrophobic reagents for glycan analysis using the correlation between hydrophobicity and theoretical non-polar surface area calculation to facilitate the development of an optimum tag for glycan derivatization. The compatibility and advantages of this method are demonstrated by cleaving and derivatizing N-linked glycans from human plasma proteins. The ESI-MS signal for the tagged glycans are shown to be significantly more abundant, and the detection of negatively charged sialylated glycans is enhanced.
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DOI：
10.1016/s0021-9673(00)96972-3
发表时间：
1990-01-19
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALPERT, AJ
通讯作者：
ALPERT, AJ
DOI：
10.1016/j.jchromb.2009.02.040
发表时间：
2009-04-01
影响因子：
3
作者：
Andrews, Genna L.;Shuford, Christopher M.;Burnett, John C., Jr.;Hawkridge, Adam M.;Muddiman, David C.
通讯作者：
Muddiman, David C.
影响因子：
7.4
作者：
Chai, WG;Piskarev, V;Lawson, AM
通讯作者：
Lawson, AM
DOI：
10.1016/s1044-0305(00)00156-2
发表时间：
2000-10-01
影响因子：
3.2
作者：
Harvey, DJ
通讯作者：
Harvey, DJ
影响因子：
3.1
作者：
Guillard, Mailys;Gloerich, Jolein;Lefeber, Dirk J.
通讯作者：
Lefeber, Dirk J.