Feeding Reduces Activity of Growth Hormone-Releasing Hormone and Somatostatin Neurons (44482)
Feeding Reduces Activity of Growth Hormone-Releasing Hormone and Somatostatin Neurons (44482)
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进食会降低生长激素释放激素和生长抑素神经元的活性 (44482)
DOI：
10.1177/153537020022300215
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发表时间：
2000
影响因子：
3.2
通讯作者：
H. Tucker
中科院分区：
文献类型：
--
作者：
C. Mcmahon;L. Chapin;K. Lookingland;R. Radcliff;H. Tucker
当每天只能获得2小时的饲料时，被阉割的公牛(公牛)在喂食前后生长激素(GH)的分泌是同步的。通常，生长激素浓度在喂食前增加，在喂食后减少。我们的目标是确定：i)开始喂食后血液中GH浓度降低；ii)喂食后弓状核(ARC)内GHRH-ir免疫反应性神经元的活性降低；iii)室周核(PeVN)内生长抑素(SS-ir)神经元的免疫反应性生长抑素(SS-ir)神经元的活性在喂食后增加；以及iv)GHRH在喂食1000至1200小时时刺激类似幅度的GH在0900和1300小时的释放。从上午7点到下午13点每隔20分钟采集一次血样。每组在0700、0900、1100和1300小时实施安乐死(n=5)。用Fos和Fos相关抗原抗体(Fos/FRA)作为免疫反应阳性的GHRH和SS神经元活动的标志，在下丘脑自由切片上进行双标记免疫组织化学染色。饲喂前生长激素浓度较高，饲喂后生长激素浓度下降。含Fos/FRA-ir的SS-ir神经元在1100小时较0900小时减少50%(P<；0.01)，1300小时减少36%(P<；0.05)。ARC内含Fos/FRA-ir的SS-ir神经元百分比无明显变化。ARC内含Fos/FRA-ir的GHRH-ir神经元的百分比在1100小时比0700小时低66%(P<；0.05)，1300小时比0700小时低65%(P<；0.05)。相反，GHRH-ir神经元的数量从0700小时增加到1300小时。与0900小时相比，GHRH诱导的GH释放在1300小时受到抑制。综上所述，基础和GHRH诱导的GH分泌减少与ARC内GHRH神经元活性降低和PeVN内SS神经元活性降低有关。
Secretion of growth hormone (GH) is synchronized among castrate male cattle (steers) around feeding when access to feed is restricted to a 2-hr period each day. Typically, concentrations of GH increase before and decrease after feeding. Our objectives were to determine whether i) concentrations of GH decrease in blood after start of feeding; ii) activity of immunoreactive growth hormone-releasing hormone (GHRH-ir) neurons decreases in the arcuate nucleus (ARC) after feeding; iii) activity of immunoreactive somatostatin (SS-ir) neurons in the periventricular nucleus (PeVN) and ARC increase after feeding; and iv) GHRH stimulates release of GH to a similar magnitude at 0900 and at 1300 hr, in steers fed between 1000 and 1200 hr. Blood samples were collected at 20-min intervals from 0700 to 1300 hr. Groups of steers were euthanized at 0700, 0900, 1100, and 1300 hr (n = 5 per group). Dual-label immunohistochemistry was performed on free-floating sections of hypothalami using antibodies directed against Fos and Fos-related antigens (Fos/FRA) as a marker of neuronal activity in immunoreactive GHRH and SS neurons. Concentrations of GH were high before and decreased after feeding. The percentage of SS-ir neurons containing Fos/FRA-ir in the PeVN was 50% lower (P < 0.01) at 1100 hr and 36% lower (P < 0.05) at 1300 hr than at 0900 hr. There was no change in percentage of SS-ir neurons containing Fos/FRA-ir in the ARC. The percentage of GHRH-ir neurons containing Fos/FRA-ir in the ARC was 66% lower (P < 0.05) at 1100 hr and 65% lower (P < 0.05) at 1300 hr than at 0700 hr. In contrast, the number of GHRH-ir neurons increased from 0700 to 1300 hr. GHRH-induced release of GH was suppressed at 1300 hr compared with 0900 hr. In conclusion, reduced basal and GHRH-induced secretion of GH after feeding was associated with decreased activity of GHRH neurons in the ARC and decreased activity of SS neurons in the PeVN.
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DOI：
10.1016/0006-291x(83)91664-9
发表时间：
1983
影响因子：
3.1
作者：
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DOI：
10.1073/pnas.88.23.10931
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DOI：
10.1210/endo.136.8.7628409
发表时间：
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期刊：
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影响因子：
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DOI：
10.1152/ajpcell.1995.269.3.c683
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
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作者：
Vandorpe,D;Kizer,N;Ciampollilo,F;Moyer,B;Karlson,K;Guggino,WB;Stanton,BA
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