Evidence that ventilatory rhythmogenesis in the frog involves two distinct neuronal oscillators
Evidence that ventilatory rhythmogenesis in the frog involves two distinct neuronal oscillators
复制标题
有证据表明青蛙的通气节律发生涉及两种不同的神经元振荡器
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Remmers
中科院分区：
文献类型：
--
作者：
Richard J. A. Wilson;K. Vasilakos;M. Harris;Christian Straus;J. Remmers
在牛蛙中，上呼吸道用于两种不同但高度协调的呼吸行为：口腔通气和肺充气循环。这些行为是如何产生和协调的尚不清楚。本研究的目的是确定假定的节律脑干位点参与这些行为。我们调查了分离的变性后脑干，以确定局部去极化的部位，通过显微注射非NMDA谷氨酸受体激动剂AMPA产生，增强了兴奋性运动模式。确定了两个地点：尾侧部位，在颅神经（CN）X水平，AMPA注射引起颊爆发频率增加，但消除肺爆发，和吻侧部位，在CN VIII和IX水平之间，注射增加两种类型的呼吸爆发的频率。使用GABA显微注射以局部抑制细胞进一步检查这两个部位。GABA注射到尾部网站抑制颊节奏，但肺节奏继续，虽然在不同的频率。当GABA注射到嘴部部位时，肺爆发被消除，但颊节律继续。当这两个网站物理分离横断，头端和尾侧脑干部分能够rhythmogenesis。结果表明，两栖动物脑干内的呼吸网络至少由两个不同但相互作用的振子组成，颊振子和肺振子。这些假定的振子可能为研究脊椎动物中的耦合振子提供一个有前途的实验模型。
In Rana catesbeiana the upper airways are used for two distinct yet highly coordinated ventilatory behaviours: buccal ventilation and lung inflation cycles. How these behaviours are generated and coordinated is unknown. The purpose of this study was to identify putative rhythmogenic brainstem loci involved in these ventilatory behaviours. We surveyed the isolated postmetamorphic brainstem to determine sites where local depolarization, produced by microinjecting the non‐NMDA glutamate receptor agonist, AMPA, augmented the ventilatory motor patterns. Two sites were identified: a caudal site, at the level of cranial nerve (CN) X, where AMPA injections caused increased buccal burst frequency but abolished lung bursts, and a rostral site, between the levels of CN VIII and IX, where injections increased the frequency of both types of ventilatory bursts. These two sites were further examined using GABA microinjections to locally inhibit cells. GABA injected into the caudal site suppressed the buccal rhythm but the lung rhythm continued, albeit at a different frequency. When GABA was injected into the rostral site the lung bursts were abolished but the buccal rhythm continued. When the two sites were physically separated by transection, both rostral and caudal brainstem sections were capable of rhythmogenesis. The results suggest the respiratory network within the amphibian brainstem is composed of at least two distinct but interacting oscillators, the buccal and lung oscillators. These putative oscillators may provide a promising experimental model for studying coupled oscillators in vertebrates.
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影响因子：
2.5
作者：
LUHMANN, HJ;PRINCE, DA
通讯作者：
PRINCE, DA
影响因子：
56.9
作者：
SMITH, JC;ELLENBERGER, HH;FELDMAN, JL
通讯作者：
FELDMAN, JL
影响因子：
2.5
作者：
JOHNSON, SM;SMITH, JC;FELDMAN, JL
通讯作者：
FELDMAN, JL
影响因子：
5.5
作者：
GREER, JJ;SMITH, JC;FELDMAN, JL
通讯作者：
FELDMAN, JL
蝌蚪体外脑干面核的呼吸活动。
DOI：
10.1113/jphysiol.1996.sp021327
发表时间：
1996
期刊：
The Journal of physiology
影响因子：
--
作者：
LiaoG-S;Kubin,L;Galante,RJ;Fishman,AP;Pack,AI
通讯作者：
Pack,AI