Down-regulation of the large conductance Ca2+-activated K+ channel expression in C57BL/6J cochlea
Down-regulation of the large conductance Ca2+-activated K+ channel expression in C57BL/6J cochlea
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C57BL/6J 耳蜗中大电导 Ca2 激活 K 通道表达的下调
DOI：
10.3109/00016489.2016.1168941
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发表时间：
2016-04
影响因子：
1.4
通讯作者：
Tao Yanling
中科院分区：
文献类型：
--
作者：
Pan Chunchen;Chu Hanqi;Lai Yanbing;Liu Yun;Sun Yanbo;Du Zhihui;Chen Jin;Tong Ting;Chen Qingguo;Zhou Liangqiang;Bing Dan;Tao Yanling
结论:年龄相关性听力损失(AHL)小鼠耳蜗中大电导Ca(2+)激活K(+)通道(BK)表达降低。 BK通道可能与AHL有关。 目的：AHL是老年人中最常见的一种。 BK 通道充当膜电压和细胞内 Ca(2+) 的传感器，对于听力至关重要。目的探讨BK通道在C57BL/6J小鼠耳蜗中的分布情况，以及BK通道的表达与AHL病因的关系。 方法：研究不同年龄（4、12、26、52周）C57BL/6J小鼠耳蜗中BK的表达情况。采用免疫荧光技术、Western blot和实时定量PCR检测BK在蛋白和mRNA水平的表达。结果：与4周龄小鼠相比，12、26和52周龄耳蜗中BK的蛋白和mRNA表达均显着且逐渐下降。免疫荧光技术显示BK通道位于毛细胞以及螺旋神经节、螺旋韧带和血管纹的细胞中，其表达量也随着年龄的增长而减少。
Conclusion:The large conductance Ca(2+)-activated K(+ )channels (BK) expression is decreased in the cochleae of age-related hearing loss (AHL) mice. BK channel may be associated with AHL.Objective:AHL is the most common among elderly persons. BK channels act as sensors for membrane voltage and intracellular Ca(2+ )and are essential for hearing. To investigate the distribution of BK channel in the cochleae of C57BL/6J mice, and the relationship between the expression of BK channel and the etiology of AHL.Methods:BK expression was studied in the cochleae of C57BL/6J mice at various ages (4, 12, 26, 52 weeks). The expressions of BK at the protein and mRNA levels were detected by immunofluorescence technique, western blot and quantitative real time PCR.Results:In comparison with 4-week-old mice, BK expressions in the cochleae at 12, 26 and 52 weeks of age were significantly and gradually decreased at both the protein and the mRNA levels. The immunofluorescence technique showed the BK channel was located in the hair cells and cells of the spiral ganglion, spiral ligament and stria vascularis and its expression also decreased with aging.
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DOI：
--
发表时间：
2004
影响因子：
11.1
作者：
L. Rüttiger;Matthias Sausbier;U. Zimmermann;Harald Winter;Claudia Braig;J. Engel;Martina Knirsch;C. Arntz;Patricia Langer;B. Hirt;Marcus Müller;I. Köpschall;M. Pfister;S. Münkner;K. Rohbock;I. Pfaff;A. Rüsch;P. Ruth;M. Knipper
通讯作者：
L. Rüttiger;Matthias Sausbier;U. Zimmermann;Harald Winter;Claudia Braig;J. Engel;Martina Knirsch;C. Arntz;Patricia Langer;B. Hirt;Marcus Müller;I. Köpschall;M. Pfister;S. Münkner;K. Rohbock;I. Pfaff;A. Rüsch;P. Ruth;M. Knipper
影响因子：
2.8
作者：
Ichimiya, I;Suzuki, M;Mogi, G
通讯作者：
Mogi, G
影响因子：
7
作者：
Kathiresan, Thandavarayan;Harvey, Margaret;Sokolowski, Bernd
通讯作者：
Sokolowski, Bernd
影响因子：
2.8
作者：
Yamasoba T;Lin FR;Someya S;Kashio A;Sakamoto T;Kondo K
通讯作者：
Kondo K
DOI：
--
发表时间：
2004
期刊：
--
影响因子：
--
作者：
L. Rüttiger;Matthias Sausbier;U. Zimmermann;Harald Winter;Claudia Braig;J. Engel;Martina Knirsch;C. Arntz;Patricia Langer;B. Hirt;Marcus Müller;I. Köpschall;M. Pfister;S. Münkner;K. Rohbock;I. Pfaff;A. Rüsch;P. Ruth;M. Knipper
通讯作者：
L. Rüttiger;Matthias Sausbier;U. Zimmermann;Harald Winter;Claudia Braig;J. Engel;Martina Knirsch;C. Arntz;Patricia Langer;B. Hirt;Marcus Müller;I. Köpschall;M. Pfister;S. Münkner;K. Rohbock;I. Pfaff;A. Rüsch;P. Ruth;M. Knipper