Time Series Tracking of Cerebral Microvascular Adaptation to Hypoxia and Hyperoxia Imaged with Repeated In Vivo Two-Photon Microscopy.
Time Series Tracking of Cerebral Microvascular Adaptation to Hypoxia and Hyperoxia Imaged with Repeated In Vivo Two-Photon Microscopy.
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通过体内双光子显微镜重复成像脑微血管对缺氧和高氧适应的时间序列跟踪。
DOI：
10.1007/978-3-030-48238-1_51
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Masamoto K.
中科院分区：
文献类型：
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作者：
Sugashi T.;Niizawa T.;Suzuki H.;Takuwa H.;Unekawa M.;Tomita Y.;Kanno I.;Masamoto K.
本研究描述了使用长期双光子显微镜采集的血管造影图像数据的图像分析方法学方面，用于研究小鼠大脑皮质中毛细血管（直径小于8 μm）的三维（3D）网络结构的动态变化。体积图像相同的毛细血管在不同时期的天长达32天的适应下，无论是慢性缺氧（8- 9%O2）或高氧（40- 50%O2）进行了比较。结果表明，缺氧0、1、2、3周时毛细血管中位直径分别为5.8、8.4、9.0、8.4 μm（N= 1，第0天n= 2193对），以及在高氧下每周测量5.4、5.7、5.4、6.0和6.1 μm，直至32天（N= 1，第0天n= 1025对）。根据毛细血管直径的这些变化，还观察到组织空间在缺氧下以深度依赖性方式变化，但不高氧。本发明的方法为我们提供了一种借助于计算机辅助图形用户界面定量确定三维血管和组织形态的方法，该方法有助于脑微血管的形态测定分析及其与微血管同时成像的脑细胞的适应性的相关性。
The present study describes methodological aspects of image analysis for angiographic image data with long-term two-photon microscopy acquired for the investigation of dynamic changes in the three-dimensional (3D) network structure of the capillaries (less than 8 μm in diameter) in the mouse cerebral cortex. Volume images of the identical capillaries over different periods of days up to 32 days were compared for adaptation under either chronic hypoxia (8–9% O2) or hyperoxia (40–50% O2). We observed that the median diameters of measured capillaries were 5.8, 8.4, 9.0, and 8.4 μm at 0, 1, 2, and 3 weeks during exposure to hypoxia, respectively (N= 1,n= 2193 pairs at day 0), and 5.4, 5.7, 5.4, 6.0, and 6.1 μm measured weekly up to 32 days under hyperoxia (N= 1,n= 1025 pairs at day 0). In accordance with these changes in capillary diameters, tissue space was also observed to change in a depth-dependent manner under hypoxia, but not hyperoxia. The present methods provide us with a method to quantitatively determine three-dimensional vascular and tissue morphology with the aid of a computer-assisted graphical user interface, which facilitates morphometric analysis of the cerebral microvasculature and its correlation with the adaptation of brain cells imaged simultaneously with the microvasculature.
影响因子：
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作者：
K. Yoshihara;H. Takuwa;I. Kanno;S. Okawa;Yukio Yamada;K. Masamoto
通讯作者：
K. Yoshihara;H. Takuwa;I. Kanno;S. Okawa;Yukio Yamada;K. Masamoto
对用双光子显微镜捕获的脑穿通动脉和静脉的直径和分支点进行自动图像分析。
影响因子：
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作者：
Takuma Sugashi;K. Yoshihara;H. Kawaguchi;H. Takuwa;Hiroshi Ito;I. Kanno;Yukio Yamada;K. Masamoto
通讯作者：
K. Masamoto