Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide.
Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide.
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β3 亚基的分子克隆，β3 亚基是 G 蛋白 β 亚基多肽的第三种形式。
DOI：
10.1073/pnas.87.6.2329
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发表时间：
1990
影响因子：
11.1
通讯作者：
Ahn,TG
中科院分区：
文献类型：
--
作者：
Levine,MA;Smallwood,PM;MoenJr,PT;Helman,LJ;Ahn,TG
信号转导鸟嘌呤核苷酸结合调节（G）蛋白是由三个亚基-α，β和γ组成的异源三聚体。虽然已经描述了多种不同形式的α亚基，但G蛋白的β亚基只有两种形式。为了进一步研究β亚基的结构多样性，我们筛选了牛和人视网膜cDNA文库，并分离出编码三种不同类型G蛋白β亚基的克隆。一种形式与先前分离的编码36 kDa形式β亚基的β 1亚基cDNA克隆相同，而第二种形式与先前描述的编码35 kDa β同种型的β 2 cDNA相同。此外，我们还发现了另一种被命名为β 3亚基的物种，它编码β亚基的第三种不同形式。β 3-亚基cDNA对应于在检查的所有组织和克隆细胞系中表达的2.0-脱氢酶mRNA。核苷酸序列分析表明，编码肽由340个氨基酸残基组成，Mr为37，221。三个β亚基的氨基酸序列密切相关：β 1和β 3亚基之间的同一性为83%，β 2和β 3亚基之间的同一性为81%。与此相反，3个cDNA的3 '非翻译区没有显示出显著的同源性。我们的数据支持的假设，一个家庭的β-亚基多肽的存在和扩展的β-亚基结构的理解。
The signal-transducing guanine nucleotide-binding regulatory (G) proteins are heterotrimers composed of three subunits--alpha, beta, and gamma. Although multiple distinctive forms of the alpha subunit have been described, only two forms of the beta subunits of the G proteins have been identified. To investigate further the structural diversity of the beta subunits, we screened bovine and human retina cDNA libraries and isolated clones encoding three distinct types of G protein beta subunit. One form was identical to previously isolated beta 1-subunit cDNA clones that encode the 36-kDa form of the beta subunit, whereas a second form was identical to previously described beta 2 cDNAs that encode the 35-kDa beta isoform. In addition, we identified another species, designated beta 3 subunit, which encodes a third distinct form of the beta subunit. The beta 3-subunit cDNA corresponds to a 2.0-kilobase mRNA expressed in all tissues and clonal cell lines examined. Nucleotide sequence analysis indicates that the encoded peptide consists of 340-amino acid residues with a Mr of 37,221. The amino acid sequences of the three beta subunits are closely related: 83% identity between beta 1 and beta 3 subunits and 81% identity between beta 2 and beta 3 subunits. By contrast, the 3'-untranslated regions of the three cDNAs show no significant homology. Our data support the hypothesis that a family of beta-subunit polypeptides exists and extend understanding of beta-subunit structure.
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马萨诸塞州总医院
DOI：
10.4135/9781483347660.n268
发表时间：
1850
期刊：
The Buffalo Medical Journal and Monthly Review of Medical and Surgical Science
影响因子：
--
作者：
Fernando Herrera
通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1176/ajp.143.8.982
发表时间：
1986-08
期刊：
The American journal of psychiatry
影响因子：
--
作者：
J. Holland;A. Korzun;S. Tross;P. Silberfarb;M. Perry;R. Comis;M. Oster
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影响因子：
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作者：
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儿科癌症幸存者的明显身体损伤和心理调整。
影响因子：
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作者：
J. E. O'Malley;D. Foster;G. Koocher;L. Slavin
通讯作者：
L. Slavin