The First Two Complete Mitochondrial Genomes for the Subfamily Meligethinae (Coleoptera: Nitidulidae) and Implications for the Higher Phylogeny of Nitidulidae.
The First Two Complete Mitochondrial Genomes for the Subfamily Meligethinae (Coleoptera: Nitidulidae) and Implications for the Higher Phylogeny of Nitidulidae.
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Nitidulidae科的系统发育状况及其姊妹群关系仍然存在争议。此外，该亚科的系统发育状况及其与Nitidulidae其他亚科的系统发育关系尚不完全清楚。线粒体基因组序列可用于研究物种鉴定、系统发育和群体遗传结构，并为进一步的遗传研究提供有价值的分子标记。本文首次提供了Meligethinae的两个线粒体全基因组，并在此基础上探讨了Nitidulidae科和Meligethinae亚科的系统发育状况。比较分析了楚塞Meligethinus tschungseni和亲和芸苔（Brassicogethes affinis）线粒体全基因组的一般特征和非编码区模式，发现两者的碱基组成和线粒体基因组结构存在显著差异。基于20个线粒体基因组的系统发育分析结果，讨论了Nitidulidae及其姊妹类群的地位。我们还试图分析Meligethinae的分类地位及其姊妹群关系。本研究将为进一步研究牛虻科高等系统发育提供基础。Nitidulidae科的系统发育状况及其姊妹群关系仍然存在争议。此外，Meligethinae亚科的地位尚不完全清楚，以往的研究主要基于形态学、分子片段和生物习性，而不是对线粒体全基因组的分析。到目前为止，还没有完整的Meligethinae线粒体基因组的报道。本研究提供了来自中国的Meligethinus tschungseni和Brassicogethes affinis的线粒体全基因组，并与现有的Nitidulidae的线粒体全基因组进行了比较。通过系统贝叶斯分析和最大似然分析对20种鞘翅目昆虫的系统发育进行了重建。结果表明，该植物的总长度分别为15783 bp和16622 bp， AT含量分别为77%和76.7%。每个线粒体全基因组包含13个蛋白质编码基因（PCGs）、22个转移RNA基因（tRNAs）、2个核糖体RNA基因（rrna）和一个控制区（a + t富区）。所有的pcg都以标准的起始密码子ATN （ATA， ATT， ATG， ATC）开头。除了cox1、cox2、nad4和nad5以单个t结尾外，所有的PCGs都以一个完整的末端密码子TAA或TAG结尾。此外，除了trnS1外，所有的trna都具有典型的三叶草二级结构，trnS1的东华大学臂在两个物种中都缺失。两个新测序的物种在对照区串联重复区数目和长度不同。基于遗传距离和Ka/Ks分析，nad6表现出更高的变异性和更快的进化速度。结果表明，Nitidulidae的4个亚科（Nitidulinae、Meligethinae、Carpophilinae、Epuraeinae）形成了一个单系群，进一步支持了Nitidulidae + Kateretidae的姐妹群关系。此外，还对Meligethinae的分类地位以及Meligethinae与Nitidulinae（目前限定的Nitidulinae）的姊妹类群关系进行了初步探讨。
The phylogenetic status of the family Nitidulidae and its sister group relationship remain controversial. Also, the phylogenetic status of the subfamily Meligethinae and its phylogenetic relationships with other subfamilies of Nitidulidae are not fully understood. Mitochondrial genome sequences can be used to study species identification, phylogeny, and population genetic structure, and to provide valuable molecular markers for further genetic studies. In this paper, two complete mitochondrial genomes of Meligethinae were provided for the first time, and the phylogenetic status of the family Nitidulidae and subfamily Meligethinae were explored based on the complete mitochondrial genomes. A comparative analysis of the general characteristics and non-coding region patterns of the complete mitochondrial genomes of Meligethinus tschungseni and Brassicogethes affinis revealed that the base composition and mitochondrial genome structure of these two species are markedly different. Given the results of the phylogenetic analysis based on 20 mitochondrial genomes, the status of Nitidulidae and its sister group relationship is discussed. We also attempted to analyze the taxonomic status of Meligethinae and its sister group relationship. This study will provide a basis for further studies on the higher phylogeny of Nitidulidae. The phylogenetic status of the family Nitidulidae and its sister group relationship remain controversial. Also, the status of the subfamily Meligethinae is not fully understood, and previous studies have been mainly based on morphology, molecular fragments, and biological habits, rather than the analysis of the complete mitochondrial genome. Up to now, there has been no complete mitochondrial genome report of Meligethinae. In this study, the complete mitochondrial genomes of Meligethinus tschungseni and Brassicogethes affinis (both from China) were provided, and they were compared with the existing complete mitochondrial genomes of Nitidulidae. The phylogenetic analysis among 20 species of Coleoptera was reconstructed via PhyloBayes analysis and Maximum likelihood (ML) analysis, respectively. The results showed that the full lengths of Meligethinus tschungseni and Brassicogethes affinis were 15,783 bp and 16,622 bp, and the AT contents were 77% and 76.7%, respectively. Each complete mitochondrial genome contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and a control region (A + T-rich region). All the PCGs begin with the standard start codon ATN (ATA, ATT, ATG, ATC). All the PCGs terminate with a complete terminal codon, TAA or TAG, except cox1, cox2, nad4, and nad5, which terminate with a single T. Furthermore, all the tRNAs have a typical clover-leaf secondary structure except trnS1, whose DHU arm is missing in both species. The two newly sequenced species have different numbers and lengths of tandem repeat regions in their control regions. Based on the genetic distance and Ka/Ks analysis, nad6 showed a higher variability and faster evolutionary rate. Based on the available complete mitochondrial genomes, the results showed that the four subfamilies (Nitidulinae, Meligethinae, Carpophilinae, Epuraeinae) of Nitidulidae formed a monophyletic group and further supported the sister group relationship of Nitidulidae + Kateretidae. In addition, the taxonomic status of Meligethinae and the sister group relationship between Meligethinae and Nitidulinae (the latter as currently circumscribed) were also preliminarily explored.
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影响因子：
4.8
作者：
Cline, Andrew R.;Smith, Trevor R.;Audisio, Paolo
通讯作者：
Audisio, Paolo
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
DOI：
10.1128/genomea.01165-17
发表时间：
2017-11-02
期刊：
Genome announcements
影响因子：
--
作者：
Duquesne V;Delcont A;Huleux A;Beven V;Touzain F;Ribière-Chabert M
通讯作者：
Ribière-Chabert M
影响因子：
4.8
作者：
Jin, Mengjie;Zwick, Andreas;Pang, Hong
通讯作者：
Pang, Hong
罗夫甲虫亚科 Staphylininae（鞘翅目：Staphylinidae）形态学和分子系统发育的一致性
影响因子：
4.6
作者：
Cai, Chen-Yang;Wang, Yong-Li;Zhou, Yu-Lingzi
通讯作者：
Zhou, Yu-Lingzi