Assessment and comparison of magnetic nanoparticles as MRI contrast agents in a rodent model of human hepatocellular carcinoma.
Assessment and comparison of magnetic nanoparticles as MRI contrast agents in a rodent model of human hepatocellular carcinoma.
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DOI：
10.1002/cmmi.494
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发表时间：
2012-07
影响因子：
--
通讯作者：
Chen, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Bu, Lihong;Xie, Jin;Chen, Kai;Huang, Jing;Aguilar, Zoraida P.;Wang, Andrew;Sun, Kin Wai;Chua, Mei-Sze;So, Samuel;Cheng, Zhen;Eden, Henry S.;Shen, Baozhong;Chen, Xiaoyuan
关键词：
本研究的目的是合成、表征和定制具有生物相容性共聚物涂层的磁性纳米粒子的表面特性，并评估所得纳米缀合物作为肝脏成像磁共振成像（MRI）造影剂的效率。通过热解合成了核心直径为 10 和 30 nm 的磁性纳米粒子，随后用含有羧基 (SHP) 或甲氧基 (SMG) 作为末端的共聚物进行涂覆。将所有四种配方和铁氧化物 (Feridex I.V.®) 分别静脉注射到单独的正常 Balb/C 小鼠中（2.5、1.0 和 0.56 mg Fe/kg），并在注射后 (p.i.) 的多个时间点对动物进行 T2 加权 MRI，以评估肝脏的摄取和清除。此外，我们还比较了新配方和 Feridex 在正交异性 Huh7 肿瘤模型中检测肿瘤的能力。 TEM 显示 10 nm 和 30 nm 纳米颗粒的尺寸分布较窄，而 Feridex 的尺寸分布较宽。 MTT、细胞凋亡和Cyclin/DNA流式细胞仪检测表明聚合物包覆的纳米颗粒对细胞生长没有不利影响。在包括 Feridex 在内的所有测试配方中，SHP-30 在体外水平显示出最高的巨噬细胞摄取率。对正常小鼠的体内 MRI 研究证实了 SHP-30 在诱导肝组织低信号方面的优越性，特别是在临床剂量（0.56 mg Fe/kg）和 3T 场下。在正交各向异性 Huh7 肿瘤模型上，SHP-30 显示出比 Feridex 更好的对比噪声比 (CNR)。 SHP-30 被发现是一种有效的肝脏 MR 成像造影剂。这项研究的成功表明，通过改进合成方法并调整 IONP 的表面特性，人们可以得到比 Feridex 更好的配方用于临床实践。
The purpose of this study was to synthesize, characterize and tailor the surface properties of magnetic nanoparticles with biocompatible copolymer coatings and to evaluate the efficiency of the resulting nanoconjugates as magnetic resonance imaging (MRI) contrast agents for liver imaging. Magnetic nanoparticles with core diameters of 10 and 30 nm were synthesized by pyrolysis and were subsequently coated with a copolymer containing either carboxyl (SHP) or methoxy groups (SMG) as termini. All four formulas, and ferumoxides (Feridex I.V.®), were individually injected intravenously into separate, normal Balb/C mice (at 2.5, 1.0, and 0.56 mg Fe/kg), and the animals underwent T2-weighted MRI at multiple time points post injection (p.i.) to evaluate the hepatic uptake and clearance. Furthermore, we compared the abilities of the new formulas and Feridex to detect tumors in an orthotropic Huh7 tumor model. TEM revealed a narrow size distribution of both the 10 nm and 30 nm nanoparticles, in contrast to a wide size distribution of Feridex. MTT, apoptosis and Cyclin/DNA flow cytometry assays showed that the polymer coated nanoparticles had no adverse effect on cell growth. Among all the tested formulas, including Feridex, SHP-30 showed the highest macrophage uptake at the in vitro level. In vivo MRI studies on normal mice confirmed the superiority of SHP-30 in inducing hypointensities in the liver tissue, especially at clinical dose (0.56 mg Fe/kg) and 3T field. SHP-30 showed better contrast-to-noise ratio (CNR) than Feridex on the orthotropic Huh7 tumor model. SHP-30 was found to be an efficient contrast agent for liver MR imaging. The success of this study suggests that by improving the synthetic approach and by tuning the surface properties of IONPs, one can arrive at better formulas than Feridex for clinical practice.
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影响因子：
14
作者：
Maeng, Jin Hee;Lee, Don-Haeng;Hong, Soon-Sun
通讯作者：
Hong, Soon-Sun
影响因子：
16.6
作者：
Park, J;Lee, E;Hyeon, T
通讯作者：
Hyeon, T
体内蛋白酶表达的实时视频成像
影响因子：
12.4
作者：
Zhu L;Xie J;Swierczewska M;Zhang F;Quan Q;Ma Y;Fang X;Kim K;Lee S;Chen X
通讯作者：
Chen X
影响因子：
19.7
作者：
Ward, J;Guthrie, JA;Robinson, PJ
通讯作者：
Robinson, PJ
影响因子：
3.7
作者：
Yallapu, Murali Mohan;Foy, Susan P.;Jain, Tapan K.;Labhasetwar, Vinod
通讯作者：
Labhasetwar, Vinod