The extrachromosomal East protein of Drosophila can associate with polytene chromosomes and regulate gene expression.
The extrachromosomal East protein of Drosophila can associate with polytene chromosomes and regulate gene expression.
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果蝇的外染色体东蛋白可以与聚染色体相关并调节基因表达。
DOI：
10.1371/journal.pone.0000412
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发表时间：
2007-05-02
期刊：
影响因子：
3.7
通讯作者：
Chia, William
中科院分区：
文献类型：
--
作者：
Wasser, Martin;Chia, William
果蝇的EAST蛋白是细胞核可扩展的染色体外结构域的组成部分。为了更好地理解其功能，我们研究了GFP标记的EAST的动力学和定位。在活的幼虫唾液腺中，EAST-GFP是高度移动的并且定位于染色体外核质。当这些细胞被透化时，EAST-GFP迅速与多线染色体结合。随着盐浓度的降低，对染色质的亲和力增加，迁移率降低。删除EAST的C-末端残基1535至2301强烈降低了对多线染色体的亲和力。大量的EAST-GFP与异染色质共定位，并且不存在于转录活性染色体区域。EAST-GFP的主要染色体定位可以在非去污剂处理的脓疱唾液腺中检测到，因为它们经历了细胞凋亡，但在发育的早期阶段没有。与这种染色体定位模式相一致，遗传证据表明EAST在抑制基因表达中的作用，因为致命的East突变与白斑病的可行突变抑制基因是等位的。我们建议，EAST作为一个离子传感器，调节基因表达，以响应细胞内离子浓度的变化。
The EAST protein of Drosophila is a component of an expandable extrachromosomal domain of the nucleus. To better understand its function, we studied the dynamics and localization of GFP-tagged EAST. In live larval salivary glands, EAST-GFP is highly mobile and localizes to the extrachromosomal nucleoplasm. When these cells are permeabilized, EAST-GFP rapidly associated with polytene chromosomes. The affinity to chromatin increases and mobility decreases with decreasing salt concentration. Deleting the C-terminal residues 1535 to 2301 of EAST strongly reduces the affinity to polytene chromosomes. The bulk of EAST-GFP co-localizes with heterochromatin and is absent from transcriptionally active chromosomal regions. The predominantly chromosomal localization of EAST-GFP can be detected in non-detergent treated salivary glands of pupae as they undergo apoptosis, however not in earlier stages of development. Consistent with this chromosomal pattern of localization, genetic evidence indicates a role for EAST in the repression of gene expression, since a lethal east mutation is allelic to the viable mutation suppressor of white-spotted. We propose that EAST acts as an ion sensor that modulates gene expression in response to changing intracellular ion concentrations.
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影响因子：
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作者：
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影响因子：
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影响因子：
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