One-step characterization of triacylglycerols from animal fat by MALDI-TOF MS

One-step characterization of triacylglycerols from animal fat by MALDI-TOF MS
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利用 MALDI-TOF MS 对动物脂肪中的三酰甘油进行一步表征

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发表时间：
2007
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通讯作者：
F. Addeo
F. Addeo
中科院分区：
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文献类型：
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作者：
G. Picariello;R. Sacchi;F. Addeo

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利用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法（MS）对乳脂、猪油和牛脂三酰基甘油（TAG）进行了比较表征。样品形成了特征模式，主要TAG信号的数量和强度根据脂肪的不同而不同。在乳脂中，短链脂肪酸（c4 - c10）的显著贡献将TAG数扩展到c20 - c60范围。在猪油和牛油中，碳12 -碳18脂肪酸限制在碳48 -碳54，也是典型的植物油。来自反刍动物的脂肪含有猪油中缺失的奇怪TAG。识别每个动物脂肪的特征标签。c20 - c46特异性指纹乳脂；52:5、54:5、54:6 TAG标记猪油；55:0, 55:1, 55:2和54:0 TAG是牛脂的典型代表。脂肪在氢化或溴化后也进行了分析；氢化有助于识别低丰度的长链TAG，并将短链天然TAG与碰撞诱导的片段区分出来；溴化可以使饱和TAG和不饱和TAG清晰分离。通过氢化前体的源后衰变分析，鉴定了不同结构的同源等压标签脂肪酸组成的差异。MALDI-TOF已被证明可以同时检测不同TAG类别中不同不饱和度的TAG （Cn）。这些数据提供了对动物脂肪分子分化的深入了解，将分析描述提高到一个新的水平，证明了MALDI-TOF MS在这一领域迄今被低估的能力。
Comparative characterization of milk fat, lard, and beef tallow triacylglycerols (TAG) has been achieved by using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS). The samples formed characteristic patterns, with major TAG signals differing in quantity and intensity according to the fat. In milk fat, the significant contribution of short-chain fatty acids (C 4 -C 10 ) extends the TAG number to the C 20 -C 60 range. In lard and beef tallow, C 12 -C 18 fatty acids restrict the range to C 48 -C 54 , also typical of vegetable oils. Fats originating from ruminants contain odd TAG missing in lard. Signature TAG were identified for each animal fat. C 20 -C 46 specifically fingerprint milk fat; 52:5, 54:5 and 54:6 TAG mark lard; and 55:0, 55:1, 55:2 and 54:0 TAG typify beef tallow. Fats were also analyzed after hydrogenation or bromination; hydrogenation helped to fingerprint the low-abundant long-chain TAG and to distinguish short-chain native TAG from collision-induced fragments; bromination allowed clear separation of saturated and unsaturated TAG. Differences in the fatty acid composition amongst homologous isobaric TAG of different structures were identified by post-source decay analysis of hydrogenated precursors. MALDI-TOF has proven advantageous for simultaneously detecting TAG at various unsaturation degrees within different TAG classes (Cn). The data provide insight into animal fat differentiation on a molecular basis, increasing the analytical description to a new level, proving the so far underestimated capacity of MALDI-TOF MS in this field.