Genome-wide analysis of Dof family transcription factors and their responses to abiotic stresses in Chinese cabbage.
Genome-wide analysis of Dof family transcription factors and their responses to abiotic stresses in Chinese cabbage.
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DOI：
10.1186/s12864-015-1242-9
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发表时间：
2015-01-31
期刊：
影响因子：
4.4
通讯作者：
Xiong AS
中科院分区：
文献类型：
--
作者：
Ma J;Li MY;Wang F;Tang J;Xiong AS
大白菜是一种重要的叶菜类蔬菜，经历了长期的栽培和人工选择。Dof（DNA-binding One Zinc Finger）转录因子是植物特异性转录因子家族的成员，具有高度保守的Dof结构域，在植物的许多生物学过程中发挥重要作用。Dof家族转录因子是高等植物中最重要的转录调节因子家族之一，参与植物生长发育和对非生物胁迫的应答。本研究为深入了解Dof转录因子对非生物胁迫的响应机制以及这些基因在植物耐逆机制中的相互作用网络提供了理论依据。本研究对大白菜Dof家系因子进行了综合分析。共从大白菜中鉴定出76个编码BraDof家族转录因子的基因，将其分为9类。根据Dof氨基酸序列比对发现了15个基序。对BraDof家族基因的染色体定位和基因重复进行了分析。在大白菜染色体上发现了10个BraDof基因的重复事件。BraDof基因在芸苔属植物染色体上的不均匀分布可能导致BraDof基因的扩增。在Dof家族中，大白菜与拟南芥、大白菜与水稻之间分别鉴定出37个和7个直向同源基因。构建了大白菜Dof因子的互作网络。为进一步了解BraDof家族转录因子在“鲁白三号”和“青岛87- 114”两个大白菜品种中的功能和调控机制，采用实时荧光定量PCR技术，对来自9个不同类群的9个基因在4种非生物胁迫（冷、热、盐和干旱）下的表达谱进行了研究。对大白菜基因组中的Dof家族转录因子进行了分析。染色体定位结果表明，重复可能导致扩增。阐明了大白菜品种对非生物胁迫的反应。这些结果为了解BraDof基因的应激反应提供了新的见解，并促进了对大白菜Dofs结构和功能的更好了解。本文的在线版本（doi：10.1186/s12864-015-1242-9）包含补充材料，可供授权用户使用。
Chinese cabbage is an important leaf vegetable that experienced long-term cultivation and artificial selection. Dof (DNA-binding One Zinc Finger) transcription factors, with a highly conserved Dof domain, are members of a major plant-specific transcription factor family that play important roles in many plant biological processes. The Dof family transcription factors, one of the most important families of transcriptional regulators in higher plants, are involved in massive aspects of plant growth, development, and response to abiotic stresses. Our study will supply resources for understanding how Dof transcription factors respond to abiotic stress and the interaction network of these genes in tolerance mechanism. In this study, we performed a comprehensive analysis of Dof family factors in Chinese cabbage. In total, 76 genes encoding BraDof family transcription factor were identified from Chinese cabbage, and those BraDof factors were divided into nine classes. Fifteen motifs were found based on Dof amino acid sequence alignments. Chromosome locations and gene duplications of BraDof family genes were also analyzed. Ten duplicate events of BraDof genes were discovered in Chinese cabbage chromosomes. The uneven distribution of BraDof genes in Brassica chromosomes may cause the expansion of BraDof genes. In the Dof family, 37 and 7 orthologous genes were identified between Chinese cabbage and Arabidopsis and between Chinese cabbage and Oryza sativa, respectively. The interaction networks of Dof factors in Chinese cabbage were also constructed. Expression profiles of nine selected genes from different nine classes subjected to four abiotic stresses (cold, heat, salt and drought) were further investigated by quantitative real-time PCR to obtain a better understanding of the functions and regulation mechanisms of BraDof family transcription factors in two Chinese cabbage varieties, ‘Lubaisanhao’ and ‘Qingdao 87-114’. Dof-family transcription factors were analyzed in genome of Chinese cabbage. Chromosomal locations showed that duplication might result in expansion. Response to abiotic stresses was elucidated in Chinese cabbage varieties. The results provide novel insights into the stress responses of BraDof genes and promote a better understanding of the construction and function of Dofs in Chinese cabbage. The online version of this article (doi:10.1186/s12864-015-1242-9) contains supplementary material, which is available to authorized users.
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