Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress.
Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress.
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高盐胁迫下条斑菜脱落酸的合成及其抗氧化酶基因表达的调控
DOI：
10.3389/fmicb.2021.775710
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Yang J;Gu W;Feng Z;Yu B;Niu J;Wang G
脱甲酸（ABA）被认为对植物适应水限制条件至关重要，并且它在进化上的作用。因此，对ABA的合成及其对Neopropia Yezoensis的下游应力响应基因的调节的见解，Yezoensis是一种在潮间带中分布的典型古细胞膜，将改善有关ABA信号在植物中如何演变的知识。在这里，在存在外源ABA和ABA前体合成的两个特定抑制剂的情况下确定ABA含量，抗氧化酶活性和靶基因的表达的变化。在两种抑制剂的每种或组合的处理下，ABA含量下调。抗氧化剂酶活性（如SOD，CAT和APX）通过抑制剂略微降低，但在添加外源性ABA时会上调。使用实时PCR（QRT-PCR）结果的定量测定与酶活性一致。所有结果都表明，ABA还可以减轻陆孢子菌中的氧化应激。结合转录组测定法，假设ABA通过一条与高等植物中类胡萝卜素途径相似的途径在尼索氏乳杆菌中合成，MVA和MVA和MEP的MEP途径可能同时存在。还从进化的角度分析了N. Yezoensis中的ABA信号通路，并说明该藻类中ABA信号通路的出现是祖先的。另外，抗氧化酶基因启动子区域中ABRE基序的存在表明抗氧化酶系统受ABA信号通路调节。
Abscisic acid (ABA) is regarded as crucial for plant adaptation to water-limited conditions and it functions evolutionarily conserved. Thus, insights into the synthesis of ABA and its regulation on downstream stress-responsive genes in Neopyropia yezoensis, a typical Archaeplastida distributed in intertidal zone, will improve the knowledge about how ABA signaling evolved in plants. Here, the variations in ABA contents, antioxidant enzyme activities and expression of the target genes were determined under the presence of exogenous ABA and two specific inhibitors of the ABA precursor synthesis. ABA content was down-regulated under the treatments of each or the combination of the two inhibitors. Antioxidant enzyme activities like SOD, CAT and APX were decreased slightly with inhibitors, but up-regulated when the addition of exogenous ABA. The quantitative assays using real-time PCR (qRT-PCR) results were consistent with the enzyme activities. All the results suggested that ABA can also alleviate oxidative stress in N. yezoensis as it in terrestrial plant. Combined with the transcriptome assay, it was hypothesized that ABA is synthesized in N. yezoensis via a pathway that is similar to the carotenoid pathway in higher plants, and both the MVA and that the MEP pathways for isoprenyl pyrophosphate (IPP) synthesis likely exist simultaneously. The ABA signaling pathway in N. yezoensis was also analyzed from an evolutionary standpoint and it was illustrated that the emergence of the ABA signaling pathway in this alga is an ancestral one. In addition, the presence of the ABRE motif in the promoter region of antioxidase genes suggested that the antioxidase system is regulated by the ABA signaling pathway.
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影响因子：
3.7
作者：
Han M;Heppel SC;Su T;Bogs J;Zu Y;An Z;Rausch T
通讯作者：
Rausch T
使用单 SPE 纯化和同位素稀释，通过 UPLC-MS/MS 简单、快速、同时测定受伤番茄中的多种含羧基植物激素
影响因子：
1.6
作者：
Fu, Jihong;Chu, Jinfang;Yan, Cunyu
通讯作者：
Yan, Cunyu
DOI：
10.1111/j.1438-8677.1989.tb00113.x
发表时间：
1989-11-01
期刊：
BOTANICA ACTA
影响因子：
--
作者：
HIRSCH, R;HARTUNG, W;GIMMLER, H
通讯作者：
GIMMLER, H
影响因子：
4.8
作者：
Estévez, JM;Cantero, A;León, P
通讯作者：
León, P
陆生双藻纲基因组为陆地植物进化提供见解
影响因子：
64.5
作者：
Cheng, Shifeng;Xian, Wenfei;Melkonian, Michael
通讯作者：
Melkonian, Michael