Detection of recent HIV-1 infection using a new limiting-antigen avidity assay: potential for HIV-1 incidence estimates and avidity maturation studies.
Detection of recent HIV-1 infection using a new limiting-antigen avidity assay: potential for HIV-1 incidence estimates and avidity maturation studies.
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DOI：
10.1371/journal.pone.0033328
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Parekh BS
中科院分区：
文献类型：
--
作者：
Duong YT;Qiu M;De AK;Jackson K;Dobbs T;Kim AA;Nkengasong JN;Parekh BS
需要准确和可靠的实验室方法来估计艾滋病毒-1的发病率，以确定高危人群，并确定预防工作的目标和监测预防工作。我们以前描述了一种单孔限制性抗原亲合力酶免疫测定法（LAg-亲合力EIA），以检测最近的HIV-1感染。我们在这里描述了进一步的优化和表征的LAg亲合力EIA，比较它的BED测定和双孔亲合力指数（AI）EIA。标本集包括纵向血清（n = 393），收集自来自4个队列（代表4种HIV-1亚型）的89名血清转化个体，以及来自3个不同队列（有或无TB合并感染）的AIDS患者（n=488）的血清。 市售97份HIV-1阳性标本。根据需要进行BED试验、LAg-亲合力EIA、AI-EIA和HIV血清学。对质控样品的监测表明，LAg-亲合力EIA的重现性高，动态范围内的变异系数<10%。在归一化光密度（ODn）临界值为1.0时，LAg-亲合力EIA的总体平均近发型持续时间（ω）为141天（95% CI 119-160），不同HIV-1亚型和人群中的ω相似（132 - 143天）。与通过BED测定法测量的HIV-IgG水平相比，通过LAg-亲合力EIA和AI-EIA的抗体亲合力动力学在个体和亚型之间是相似的。与BED测定相比，LAg亲合力EIA在AIDS患者中的假近期率为0.2%，而BED测定为2.9%。蛋白质印迹法的标本与增加的亲和力确认最近的HIV-1感染的准确检测。这些数据表明，LAg-亲合力EIA是一种有前景的测定方法，在不同人群和亚型中具有一致的ω。该检测方法应非常有用1）估计HIV-1的发病率在横断面标本作为HIV监测的一部分，2）确定近期感染的危险因素，3）衡量预防计划的影响，和4）研究疫苗试验期间的亲合力成熟。
Accurate and reliable laboratory methods are needed for estimation of HIV-1 incidence to identify the high-risk populations and target and monitor prevention efforts. We previously described a single-well limiting-antigen avidity enzyme immunoassay (LAg-Avidity EIA) to detect recent HIV-1 infection. We describe here further optimization and characterization of LAg-Avidity EIA, comparing it to the BED assay and a two-well avidity-index (AI) EIA. Specimen sets included longitudinal sera (n = 393), collected from 89 seroconverting individuals from 4 cohorts representing 4 HIV-1 subtypes, and sera from AIDS patients (n = 488) with or without TB co-infections from 3 different cohorts. Ninety seven HIV-1 positive specimens were purchased commercially. The BED assay, LAg-Avidity EIA, AI-EIA and HIV serology were performed, as needed. Monitoring quality control specimens indicated high reproducibility of the LAg-Avidity EIA with coefficient of variation of <10% in the dynamic range. The LAg-Avidity EIA has an overall mean duration of recency (ω) of 141 days (95% CI 119–160) at normalized optical density (ODn) cutoff of 1.0, with similar ω in different HIV-1 subtypes and populations (132 to 143 days). Antibody avidity kinetics were similar among individuals and subtypes by both the LAg-Avidity EIA and AI-EIA compared to the HIV-IgG levels measured by the BED assay. The false recent rate among individuals with AIDS was 0.2% with the LAg-Avidity EIA, compared to 2.9% with the BED assay. Western blot profiles of specimens with increasing avidity confirm accurate detection of recent HIV-1 infections. These data demonstrate that the LAg-Avidity EIA is a promising assay with consistent ω in different populations and subtypes. The assay should be very useful for 1) estimating HIV-1 incidence in cross-sectional specimens as part of HIV surveillance, 2) identifying risk factors for recent infections, 3) measuring impact of prevention programs, and 4) studying avidity maturation during vaccine trials.
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影响因子：
1.5
作者：
Parekh, BS;Kennedy, MS;McDougal, JS
通讯作者：
McDougal, JS
影响因子：
9.4
作者：
Dobbs, T;Kennedy, S;Parekh, BS
通讯作者：
Parekh, BS
DOI：
10.1097/qai.0b013e3181ab6ef0
发表时间：
2009-10-01
影响因子：
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作者：
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通讯作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
QUINN, TC