Endothelin converting enzyme-1-, endothelin-1-, and endothelin-3-like immunoreactivity in the rat brain
Endothelin converting enzyme-1-, endothelin-1-, and endothelin-3-like immunoreactivity in the rat brain
复制标题
大鼠脑中的内皮素转换酶-1-、内皮素-1-和内皮素-3样免疫反应性
DOI：
10.1016/s0306-4522(98)00692-7
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
J. Lehmann
中科院分区：
文献类型：
--
作者：
J. M. Sluck;R. Lin;L. Katolik;A. Jeng;J. Lehmann
可能使用内皮素作为神经递质/神经激素的神经元在大鼠脑中使用针对内皮素转换酶-1、内皮素-1和内皮素-3的多克隆抗体进行映射。抗内皮素转换酶-1抗体产生最稳健的染色，允许最佳可视化的神经元的分布和形态。标记神经元分布于丘脑背核、丘脑网状核、视前内侧区、脑桥核和蓝斑。内皮素转换酶样免疫反应性在蓝斑和丘脑网状核中的定位表明内皮素分别与去甲肾上腺素和GABA共定位。此外，内皮素转换酶样免疫反应被发现在苍白球，隔核，并在两个垂直和水平四肢的斜角带的Broca核，和腹外侧区的尾壳核。强烈的内皮素转换酶样免疫反应被发现在整个新皮层的锥体神经元的连续带主要在第五层，延伸到扣带回和梨状皮质。运动核团，包括眼神经，面神经和三叉神经核，也内皮素转换酶免疫反应。小脑浦肯野细胞染色。非神经元细胞，如少突胶质细胞，小胶质细胞和星形胶质细胞一般不内皮素转化酶免疫反应，虽然星形胶质细胞很少染色。内皮素转换酶，内皮素-1，内皮素-3样免疫反应通常被发现共存于给定的核。内皮素免疫染色神经元的多样性表明内皮素在中枢神经系统中的多种作用。
Neurons likely to use endothelin as a neurotransmitter/neurohormone were mapped in the rat brain using polyclonal antibodies directed against endothelin-converting enzyme-1, endothelin-1, and endothelin-3. Anti-endothelin-converting enzyme-1 antibodies produced the most robust staining, permitting the best visualization of the distribution and morphology of neurons. Labeled neurons were found in the dorsal thalamic nuclei and reticular thalamic nuclei, medial preoptic area, pontine nucleus, and locus coeruleus. Localization of endothelin-converting enzyme-like immunoreactivity in the locus coeruleus and in the reticular nucleus of the thalamus suggests that endothelin is co-localized with norepinephrine and GABA, respectively. Additionally, endothelin-converting enzyme-like immunoreactivity was found in the globus pallidus, septal nuclei, and in both the vertical and horizontal limbs of the nucleus of the diagonal band of Broca, and the ventrolateral area of the caudate–putamen. Strong endothelin-converting enzyme-like immunoreactivity was found in a continuous band of pyramidal neurons throughout the neocortex primarily in layer V, extending into the cingulate gyrus and piriform cortex. Motor nuclei, including oculomotor, facial, and trigeminal nuclei, were also endothelin-converting enzyme-immunoreactive. In the cerebellum, Purkinje cells were stained. Non-neuronal cells such as oligodendroglia, microglia, and astrocytes generally were not endothelin-converting enzyme-immunoreactive, although astrocytes were rarely stained. Endothelin-converting enzyme-, endothelin-1-, and endothelin-3-like immunoreactivities were generally found co-existing in given nuclei. The diversity of neurons immunostained for endothelin suggests multiple roles of endothelin in the CNS.
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影响因子：
4.1
作者：
Caner, HH;Kwan, AL;Lee, KS
通讯作者：
Lee, KS
影响因子：
56.9
作者：
SLOVITER, RS
通讯作者：
SLOVITER, RS
DOI：
10.1073/pnas.87.6.2359
发表时间：
1990-03-01
影响因子：
11.1
作者：
MACCUMBER, MW;ROSS, CA;SNYDER, SH
通讯作者：
SNYDER, SH
肺上皮细胞系对内皮素-1 产生的调节。
影响因子：
5.8
作者：
Calderón,E;Gómez-Sánchez,CE;Cozza,EN;Zhou,M;Coffey,RG;Lockey,RF;Prockop,LD;Szentivanyi,A
通讯作者：
Szentivanyi,A
影响因子：
4.2
作者：
SMITH, DH;SOARES, HD;MCINTOSH, TK
通讯作者：
MCINTOSH, TK