Insights into the seasonal adaptive mechanisms of Chinese alligators (Alligator sinensis) from transcriptomic analyses
Insights into the seasonal adaptive mechanisms of Chinese alligators (Alligator sinensis) from transcriptomic analyses
复制标题
从转录组学分析扬子鳄季节适应机制
DOI：
10.1071/zo18005
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发表时间：
2018-09
影响因子：
0.8
通讯作者：
Xiaobing Wu
中科院分区：
文献类型：
--
作者：
Hongji Sun;Xianbo Zuo;Long Sun;Peng Yan;Fang Zhang;Hui Xue;En Li;Yongkang Zhou;Rong Wu;Xiaobing Wu
抽象的。扬子鳄（Alligator sinensis）是中国特有的稀有物种，被认为是世界上最濒危的脊椎动物之一。众所周知，它会冬眠，这是一种应对寒冷和食物匮乏的节能策略。冬眠期间基因表达的变化仍然很大程度上未知。为了了解这些复杂的季节性适应机制，我们利用 RNA 测序对冬眠和活跃扬子鳄的心脏、骨骼肌和肾脏的差异基因表达进行了全面调查。我们总共鉴定了 4780 个在活跃期和冬眠期之间差异表达的基因。 GO 和 KEGG 通路分析表明了这些差异表达基因 (DEG) 的可能作用。活跃的扬子鳄中上调的DEG，CSRP3、MYG和PCKGC，可能分别维持心脏和骨骼肌的收缩、氧气的运输和储存，并增强身体的新陈代谢。扬子鳄休眠期间上调的DEGs ADIPO、CIRBP和TMM27可能会改善胰岛素敏感性和糖脂代谢，保护细胞免受低温和缺氧的有害影响，调节氨基酸运输和摄取，刺激胰岛细胞增殖和胰岛素分泌。这些结果为了解扬子鳄冬眠所需的季节适应的分子机制奠定了基础，也为其他非模式生物的研究提供了有效信息。
Abstract. The Chinese alligator (Alligator sinensis) is an endemic and rare species in China, and is considered to be one of the most endangered vertebrates in the world. It is known to hibernate, an energy-saving strategy against cold temperatures and food deprivation. Changes in gene expression during hibernation remain largely unknown. To understand these complex seasonal adaptive mechanisms, we performed a comprehensive survey of differential gene expression in heart, skeletal muscle, and kidney of hibernating and active Chinese alligators using RNA-Sequencing. In total, we identified 4780 genes differentially expressed between the active and hibernating periods. GO and KEGG pathway analysis indicated the likely role of these differentially expressed genes (DEGs). The upregulated DEGs in the active Chinese alligator, CSRP3, MYG and PCKGC, may maintain heart and skeletal muscle contraction, transport and storage of oxygen, and enhance the body’s metabolism, respectively. The upregulated DEGs in the dormant Chinese alligator, ADIPO, CIRBP and TMM27, may improve insulin sensitivity and glucose/lipid metabolism, protect cells against harmful effects of cold temperature and hypoxia, regulate amino acid transport and uptake, and stimulate the proliferation of islet cells and the secretion of insulin. These results provide a foundation for understanding the molecular mechanisms of the seasonal adaptation required for hibernation in Chinese alligators, as well as effective information for other non-model organisms research.
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影响因子：
3.7
作者：
Atallah J;Plachetzki DC;Jasper WC;Johnson BR
通讯作者：
Johnson BR
影响因子：
4
作者：
Shinoda, Yusuke;Yamaguchi, Masayuki;Kawaguchi, Hiroshi
通讯作者：
Kawaguchi, Hiroshi
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.