Comparative genomics defines the core genome of the growing N4-like phage genus and identifies N4-like Roseophage specific genes.
Comparative genomics defines the core genome of the growing N4-like phage genus and identifies N4-like Roseophage specific genes.
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DOI：
10.3389/fmicb.2014.00506
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发表时间：
2014
影响因子：
5.2
通讯作者：
Schäfer H
中科院分区：
文献类型：
--
作者：
Chan JZ;Millard AD;Mann NH;Schäfer H
从海水中分离到两种噬菌体RPP 1和RLP 1，它们感染海洋蔷薇属进化枝的成员。它们的线性基因组分别为74.7和74.6kb，分别编码91和92个编码DNA序列。其中约30%与肠杆菌噬菌体N4中发现的基因同源。对这两种新的Rosebellum和其他23种测序的N4-样病毒（Rosebellum谱系的3种感染成员和20种感染其他γ-变形菌）的比较基因组学显示，N4-样病毒共有14个基因的核心基因组，负责控制基因表达、复制和病毒体蛋白。这些基因的系统发育分析将五个N4样噬菌体（RN 4）放入一个不同的亚支。对RN 4噬菌体基因组的分析显示，它们共有另外19个基因，其中9个基因仅在RN 4噬菌体中发现，4个基因似乎是从它们的细菌宿主中获得的。RPP 1和RLP 1病毒体的蛋白质组学分析鉴定了RN 4噬菌体中存在的第二个结构模块，其类似于假单胞菌N4样噬菌体LIT 1中发现的结构模块。使用来自RPP 1的CDS序列对包括GOS数据库在内的各种宏基因组数据库的检索表明，这些微生物广泛分布于海洋环境中，特别是在开阔的海洋环境中。
Two bacteriophages, RPP1 and RLP1, infecting members of the marine Roseobacter clade were isolated from seawater. Their linear genomes are 74.7 and 74.6 kb and encode 91 and 92 coding DNA sequences, respectively. Around 30% of these are homologous to genes found in Enterobacter phage N4. Comparative genomics of these two new Roseobacter phages and 23 other sequenced N4-like phages (three infecting members of the Roseobacter lineage and 20 infecting other Gammaproteobacteria) revealed that N4-like phages share a core genome of 14 genes responsible for control of gene expression, replication and virion proteins. Phylogenetic analysis of these genes placed the five N4-like roseophages (RN4) into a distinct subclade. Analysis of the RN4 phage genomes revealed they share a further 19 genes of which nine are found exclusively in RN4 phages and four appear to have been acquired from their bacterial hosts. Proteomic analysis of the RPP1 and RLP1 virions identified a second structural module present in the RN4 phages similar to that found in the Pseudomonas N4-like phage LIT1. Searches of various metagenomic databases, including the GOS database, using CDS sequences from RPP1 suggests these phages are widely distributed in marine environments in particular in the open ocean environment.
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影响因子：
3.4
作者：
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通讯作者：
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