Origins of transfer establish networks of functional dependencies for plasmid transfer by conjugation.
Origins of transfer establish networks of functional dependencies for plasmid transfer by conjugation.
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转移的起源建立了质粒通过接合转移的功能依赖性网络。
DOI：
10.1093/nar/gkac1079
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发表时间：
2023-04-24
影响因子：
14.9
通讯作者：
P.C. Rocha, Eduardo
中科院分区：
文献类型：
--
作者：
Ares-Arroyo, Manuel;Coluzzi, Charles;P.C. Rocha, Eduardo
质粒可以通过接合在细胞之间转移，从而通过水平基因转移驱动细菌进化。然而，我们忽视了许多质粒转移的分子机制，因为它们缺乏接合所需的所有蛋白质编码基因。我们通过鉴定大肠杆菌和金黄色葡萄球菌中具有接合转移起源的数百个质粒和染色体解决了这个难题。这些质粒（pOriT）劫持接合或可移动元件的松弛酶，但不是两者。pOriT和其他质粒之间的功能依赖性解释了它们的共存：pOriT在具有许多质粒的细胞中是丰富的，而接合质粒在其他质粒中是最常见的。我们系统地表征了质粒迁移率与接合和转移的替代机制的关系，现在可以提出一个假定的转移机制，其中约90%。在大多数情况下，质粒移动性似乎涉及接合。有趣的是，流动性的机制是质粒编码的附属性状的重要决定因素，因为pOriTs具有最高密度的抗菌素耐药基因，而缺乏推定的转移机制的质粒具有最低的。我们阐明了质粒之间的进化关系，并建议，许多pOriT可能已经出现在其他类型的质粒的基因缺失。这些结果表明，大多数质粒可以通过接合转移。oriTs的鉴定揭示了90%质粒的迁移机制。许多以前被认为是不可传播的可能起源于较大的质粒，并依赖于功能依赖性来解释它们在细胞内的共存。
Plasmids can be transferred between cells by conjugation, thereby driving bacterial evolution by horizontal gene transfer. Yet, we ignore the molecular mechanisms of transfer for many plasmids because they lack all protein-coding genes required for conjugation. We solved this conundrum by identifying hundreds of plasmids and chromosomes with conjugative origins of transfer in Escherichia coli and Staphylococcus aureus. These plasmids (pOriT) hijack the relaxases of conjugative or mobilizable elements, but not both. The functional dependencies between pOriT and other plasmids explain their co-occurrence: pOriT are abundant in cells with many plasmids, whereas conjugative plasmids are the most common in the others. We systematically characterized plasmid mobility in relation to conjugation and alternative mechanisms of transfer and can now propose a putative mechanism of transfer for ∼90% of them. In most cases, plasmid mobility seems to involve conjugation. Interestingly, the mechanisms of mobility are important determinants of plasmid-encoded accessory traits, since pOriTs have the highest densities of antimicrobial resistance genes, whereas plasmids lacking putative mechanisms of transfer have the lowest. We illuminate the evolutionary relationships between plasmids and suggest that many pOriT may have arisen by gene deletions in other types of plasmids. These results suggest that most plasmids can be transferred by conjugation. Identification of oriTs reveals mechanisms of mobility for 90% of plasmids. Many of the previously considered non-transmissible might have originated from larger plasmids, and rely on functional dependencies that explains their co-existence within cells.
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影响因子：
5.2
作者：
Hooton SPT;Pritchard ACW;Asiani K;Gray-Hammerton CJ;Stekel DJ;Crossman LC;Millard AD;Hobman JL
通讯作者：
Hobman JL
影响因子：
3.6
作者：
Daccord, Aurelie;Ceccarelli, Daniela;Burrus, Vincent
通讯作者：
Burrus, Vincent
影响因子：
14.9
作者：
Durand R;Huguet KT;Rivard N;Carraro N;Rodrigue S;Burrus V
通讯作者：
Burrus V
DOI：
10.1073/pnas.1600974113
发表时间：
2016-07-19
影响因子：
11.1
作者：
Hall, James P. J.;Wood, A. Jamie;Brockhurst, Michael A.
通讯作者：
Brockhurst, Michael A.
影响因子：
10.7
作者：
Cury J;Oliveira PH;de la Cruz F;Rocha EPC
通讯作者：
Rocha EPC