Laboratory Stock Variants of the Archetype Silver Resistance Plasmid pMG101 Demonstrate Plasmid Fusion, Loss of Transmissibility, and Transposition of Tn7/pco/sil Into the Host Chromosome.
Laboratory Stock Variants of the Archetype Silver Resistance Plasmid pMG101 Demonstrate Plasmid Fusion, Loss of Transmissibility, and Transposition of Tn7/pco/sil Into the Host Chromosome.
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DOI：
10.3389/fmicb.2021.723322
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发表时间：
2021
影响因子：
5.2
通讯作者：
Hobman JL
中科院分区：
文献类型：
--
作者：
Hooton SPT;Pritchard ACW;Asiani K;Gray-Hammerton CJ;Stekel DJ;Crossman LC;Millard AD;Hobman JL
1973年从美国波士顿的3例烧伤病人中分离到携带多药耐药质粒pMG 101的鼠伤寒沙门氏菌。pMG 101可转移到其他沙门氏菌属。和大肠杆菌宿主，并携带一种新的和不寻常的AMR基因和银抗性的组合。pMG 101是一个183.5Kb的IncHI质粒，其中Tn 7介导的pco/sil抗性基因转座到大肠杆菌染色体上。coliK-12 J53宿主菌中发生。我们注意到两株大肠杆菌的链霉素抗性和质粒大小的差异。coli K-12 J53 pMG 101，分别来自两个不同的实验室（pMG 101-A和pMG 101-B）。两种菌株的长读序测序（PacBio）意外地揭示了两者中的质粒和染色体重排。pMG 101-A是一种非传递性383 Kb闭环质粒，由融合至IncFI/FIIA质粒的IncHI 2质粒序列组成。pMG 101-B是可移动的闭环154 K B IncFI/FIIA质粒。pMG 101-B与pMG 101-A的融合IncFI/IncFIIA区的序列同一性> 99%。组装的pMG 101-B的宿主序列读数显示Tn 7介导的pco/sil转座到E. coliJ 53染色体上。长读段序列数据结合实验室实验已经证明了pMG 101的大规模变化。接合功能的丧失和抗性基因向染色体中的移动表明，即使在长期的实验室储存下，转座子和插入序列等移动的遗传元件也可以驱动质粒和宿主的进化。这项研究强调了尽早利用质粒和宿主菌株的长读段测序技术的重要性。
Salmonella Typhimurium carrying the multidrug resistance (MDR) plasmid pMG101 was isolated from three burns patients in Boston United States in 1973. pMG101 was transferrable into other Salmonella spp. and Escherichia coli hosts and carried what was a novel and unusual combination of AMR genes and silver resistance. Previously published short-read DNA sequence of pMG101 showed that it was a 183.5Kb IncHI plasmid, where a Tn7-mediated transposition of pco/sil resistance genes into the chromosome of the E. coli K-12 J53 host strain had occurred. We noticed differences in streptomycin resistance and plasmid size between two stocks of E. coli K-12 J53 pMG101 we possessed, which had been obtained from two different laboratories (pMG101-A and pMG101-B). Long-read sequencing (PacBio) of the two strains unexpectedly revealed plasmid and chromosomal rearrangements in both. pMG101-A is a non-transmissible 383Kb closed-circular plasmid consisting of an IncHI2 plasmid sequence fused to an IncFI/FIIA plasmid. pMG101-B is a mobile closed-circular 154 Kb IncFI/FIIA plasmid. Sequence identity of pMG101-B with the fused IncFI/IncFIIA region of pMG101-A was >99%. Assembled host sequence reads of pMG101-B showed Tn7-mediated transposition of pco/sil into the E. coli J53 chromosome between yhiM and yhiN. Long read sequence data in combination with laboratory experiments have demonstrated large scale changes in pMG101. Loss of conjugation function and movement of resistance genes into the chromosome suggest that even under long-term laboratory storage, mobile genetic elements such as transposons and insertion sequences can drive the evolution of plasmids and host. This study emphasises the importance of utilising long read sequencing technologies of plasmids and host strains at the earliest opportunity.
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影响因子：
3.3
作者：
Luque, Ainara;Paytubi, Sonia;Madrid, Cristina
通讯作者：
Madrid, Cristina
DOI：
10.1016/j.ijfoodmicro.2011.08.015
发表时间：
2011-12-02
影响因子：
5.4
作者：
Hooton, Steven P. T.;Atterbury, Robert J.;Connerton, Ian F.
通讯作者：
Connerton, Ian F.
DOI：
10.1093/jac/dkaa345
发表时间：
2020-12-01
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Bortolaia V;Kaas RS;Ruppe E;Roberts MC;Schwarz S;Cattoir V;Philippon A;Allesoe RL;Rebelo AR;Florensa AF;Fagelhauer L;Chakraborty T;Neumann B;Werner G;Bender JK;Stingl K;Nguyen M;Coppens J;Xavier BB;Malhotra-Kumar S;Westh H;Pinholt M;Anjum MF;Duggett NA;Kempf I;Nykäsenoja S;Olkkola S;Wieczorek K;Amaro A;Clemente L;Mossong J;Losch S;Ragimbeau C;Lund O;Aarestrup FM
通讯作者：
Aarestrup FM
来自食品生产动物的大肠杆菌分离株的 ST3-IncHI2 质粒中金属和抗生素耐药性的共同传播。
影响因子：
4.6
作者：
Fang L;Li X;Li L;Li S;Liao X;Sun J;Liu Y
通讯作者：
Liu Y
影响因子：
11
作者：
Li, Li-Guan;Xia, Yu;Zhang, Tong
通讯作者：
Zhang, Tong