Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development.
Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development.
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玉米雄性不育33编码一种假定的甘油-3-磷酸酰基转移酶，介导花药角质层形成和小孢子发育
DOI：
10.1186/s12870-018-1543-7
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发表时间：
2018-12-03
影响因子：
5.3
通讯作者：
Jin W
中科院分区：
文献类型：
--
作者：
Zhang L;Luo H;Zhao Y;Chen X;Huang Y;Yan S;Li S;Liu M;Huang W;Zhang X;Jin W
花药角质层主要由脂质聚合物组成，对花粉发育至关重要，在高等植物有性生殖中起着重要作用。然而，玉米中脂质聚合物生物合成的机制尚不清楚。目前尚不清楚。本文报道了玉米雄性不育突变体缩小花药1 （sa1）与经典突变体雄性不育33 （ms33）等位，表现出花药角质层发育缺陷和小孢子过早降解。我们通过图谱克隆分离出MS33。MS33编码一种甘油-3-磷酸酰基转移酶，在花药发育过程中优先在绒毡层细胞中表达。气相色谱-质谱分析显示，与野生型相比，ms33花药中的蜡质和角质明显减少。因此，rna测序分析表明，与野生型相比，ms33中许多参与蜡质和角质层生物合成的基因存在差异表达。我们的研究结果表明，MS33可能通过影响玉米脂质聚酯生物合成来促进花药角质层和小孢子的发育。
The anther cuticle, which is primarily composed of lipid polymers, is crucial for pollen development and plays important roles in sexual reproduction in higher plants. However, the mechanism underlying the biosynthesis of lipid polymers in maize (Zea mays. L.) remains unclear. Here, we report that the maize male-sterile mutant shrinking anther 1 (sa1), which is allelic to the classic mutant male sterile 33 (ms33), displays defective anther cuticle development and premature microspore degradation. We isolated MS33 via map-based cloning. MS33 encodes a putative glycerol-3-phosphate acyltransferase and is preferentially expressed in tapetal cells during anther development. Gas chromatography-mass spectrometry revealed a substantial reduction in wax and cutin in ms33 anthers compared to wild type. Accordingly, RNA-sequencing analysis showed that many genes involved in wax and cutin biosynthesis are differentially expressed in ms33 compared to wild type. Our findings suggest that MS33 may contribute to anther cuticle and microspore development by affecting lipid polyester biosynthesis in maize.
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