Serum matrix metalloproteinase-3 as a noninvasive biomarker of histological synovitis for diagnosis of rheumatoid arthritis.
Serum matrix metalloproteinase-3 as a noninvasive biomarker of histological synovitis for diagnosis of rheumatoid arthritis.
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DOI：
10.1155/2014/179284
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发表时间：
2014
影响因子：
4.6
通讯作者：
Dai L
中科院分区：
文献类型：
--
作者：
Ma JD;Zhou JJ;Zheng DH;Chen LF;Mo YQ;Wei XN;Yang LJ;Dai L
目标。目的：探讨类风湿关节炎(RA)滑膜组织中基质金属蛋白酶-3(MMP-3)表达与滑膜炎的关系。方法：研究方法。采用双抗体夹心法对62例活动期类风湿关节炎患者进行血清基质金属蛋白酶-3的检测。对所有RA患者、13例骨关节炎和10例骨科关节病患者的滑膜组织切片进行苏木精-伊红染色和免疫组织化学染色，检测基质金属蛋白酶-3、CD3、CD20、CD38、CD68和CD15。结果。类风湿关节炎患者外周血中衬里MMP3+细胞的百分率明显高于对照组，且与Krenn滑膜炎症评分呈正相关(r=0.574，P<0.001)，与炎性细胞亚群呈显著正相关。多元逐步线性回归分析显示，衬里MMP3+细胞百分率与滑膜间质活化、CD68+巨噬细胞亚群和CD15+中性粒细胞亚群的相关性强于其他组织学指标。RA患者衬里MMP3+细胞百分率与血清MMP3水平呈显著正相关(r=0.656，P<0.001)。RA重度滑膜炎患者血清MMP3水平明显高于低度滑膜炎患者，且与滑膜炎症评分及滑膜基质活化评分呈显著正相关(均P<0.05)。结论。血清基质金属蛋白酶-3可作为滑膜炎组织学诊断和类风湿关节炎诊断的非侵入性生物标志物。
Objective. To explore the correlation between matrix metalloproteinase- (MMP-) 3 and histological synovitis in rheumatoid arthritis (RA). Methods. Serum MMP-3 of 62 patients with active RA was detected by ELISA. Serial synovial tissue sections from all RA patients, 13 osteoarthritis, and 10 orthopedic arthropathies patients were stained with hematoxylin and eosin and immunohistochemically for MMP-3, CD3, CD20, CD38, CD68, and CD15. Results. The percentage of lining MMP3+ cells was significantly higher in RA patients especially with high grade synovitis and it was significantly correlated with Krenn's synovitis score (r = 0.574, P < 0.001) and sublining inflammatory cells. Multivariate stepwise linear regression analysis revealed that the association of the percentage of lining MMP3+ cells with activation of synovial stroma, sublining CD68+ macrophages, and CD15+ neutrophils was stronger than other histological indicators. The percentage of lining MMP3+ cells was significantly correlated with serum MMP-3 in RA (r = 0.656, P < 0.001). Serum MMP-3 was higher in RA patients with high grade synovitis than that of low grade synovitis and significantly correlated with synovitis score and activation of synovial stroma subscore (all P < 0.05). Conclusion. Serum MMP-3 may be an alternative noninvasive biomarker of histological synovitis and RA diagnosis.
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影响因子：
2.8
作者：
Krenn, V;Morawietz, L;König, A
通讯作者：
König, A
影响因子：
27.4
作者：
OKADA, Y;TAKEUCHI, N;NAGASE, H
通讯作者：
NAGASE, H
影响因子：
27.4
作者：
Rooney, Terence;Roux-Lombard, Pascale;Bresnihan, Barry
通讯作者：
Bresnihan, Barry
影响因子：
--
作者：
ROONEY, M;CONDELL, D;BRESNIHAN, B
通讯作者：
BRESNIHAN, B
影响因子：
5.5
作者：
Roux-Lombard, P;Eberhardt, K;Wollheim, FA
通讯作者：
Wollheim, FA