Identification of Essential Genes Associated With Prodigiosin Production in Serratia marcescens FZSF02.
Identification of Essential Genes Associated With Prodigiosin Production in Serratia marcescens FZSF02.
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粘质沙雷氏菌 FZSF02 中与灵菌红生产相关的必需基因的鉴定
DOI：
10.3389/fmicb.2021.705853
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发表时间：
2021
影响因子：
5.2
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Jia X;Liu F;Zhao K;Lin J;Fang Y;Cai S;Lin C;Zhang H;Chen L;Chen J
灵芝菌素是一种很有前途的次生代谢物，主要由沙雷氏菌产生。为了研究灵芝菌素生物合成的全球调控机制，利用EZ-Tn5转座子构建了一个包含2.3万个突变克隆的突变文库，其中114个克隆显示了灵芝菌素生产能力的改变。在114个克隆中，有37个在灵芝菌素生物合成簇基因上发生了转座子插入；77个克隆中的转座子插入基因属于33个不同的外部灵芝菌素生物合成簇基因。这33个基因可分为转录调控基因、膜蛋白编码基因和代谢酶编码基因。最近有报道称，大多数基因都与灵芝菌素的生产有关。在22个不同插入基因的突变体中，PIGA基因的转录水平显著下调，这与灵芝菌素产量下降的表型一致。通过在培养基中加入甲酸尿苷(UMP)，对参与嘧啶核苷酸生物合成途径的突变基因进行了功能验证。基因互补证实了EnvZ/OmpR双组分调控系统基因envZ和ompR在灵芝菌素生产中的调控功能。
Prodigiosin is a promising secondary metabolite produced mainly by Serratia strains. To study the global regulatory mechanism of prodigiosin biosynthesis, a mutagenesis library containing 23,000 mutant clones was constructed with the EZ-Tn5 transposon, and 114 clones in the library showed altered prodigiosin production ability. For 37 of the 114 clones, transposon insertion occurred on the prodigiosin biosynthetic cluster genes; transposon inserted genes of the 77 clones belonged to 33 different outside prodigiosin biosynthetic cluster genes. These 33 genes can be divided into transcription-regulating genes, membrane protein-encoding genes, and metabolism enzyme-encoding genes. Most of the genes were newly reported to be involved in prodigiosin production. Transcriptional levels of the pigA gene were significantly downregulated in 22 mutants with different inserted genes, which was in accordance with the phenotype of decreased prodigiosin production. Functional confirmation of the mutant genes involved in the pyrimidine nucleotide biosynthesis pathway was carried out by adding orotate and uridylate (UMP) into the medium. Gene complementation confirmed the regulatory function of the EnvZ/OmpR two-component regulatory system genes envZ and ompR in prodigiosin production.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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