Serotype b of Aggregatibacter actinomycetemcomitans increases osteoclast and memory T-lymphocyte activation.
Serotype b of Aggregatibacter actinomycetemcomitans increases osteoclast and memory T-lymphocyte activation.
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伴放线聚集杆菌 b 血清型可增加破骨细胞和记忆 T 淋巴细胞的活化。
DOI：
10.1111/omi.12112
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发表时间：
2016
影响因子：
3.7
通讯作者：
R. Vernal
中科院分区：
文献类型：
--
作者：
S. Melgar;J. Díaz;C. Alvarez;L. Rojas;G. Monasterio;P. Carvajal;A. Escobar;M. Sanz;R. Vernal
在牙周炎期间，牙槽骨吸收与辅助性T细胞17型（Th 17）淋巴细胞和核因子-κB配体受体激活剂（RANKL）诱导的破骨细胞的活化相关。我们以前报道，血清型B的放线菌伴聚杆菌具有较高的能力，触发活化的T淋巴细胞的Th 17型分化和功能，其脂多糖是一个更有效的免疫原相比，其他血清型。本研究旨在研究伴放线菌血清型B是否诱导Th 17相关RANKL的产生、RANKL诱导的破骨细胞活化和抗原特异性记忆T淋巴细胞增殖。在用不同伴放线菌血清型致敏的自体树突状细胞刺激的初始CD 4（+）T淋巴细胞上，定量RANKL产生、T-bet、加塔-3、RORC 2和Foxp 3表达、RORC 2/RANKL细胞内双表达、TRAP（+）破骨细胞活化和骨吸收。在牙周炎和健康受试者中测定了响应伴放线菌血清型的增殖记忆T淋巴细胞的频率。与用其他血清型刺激的相同细胞相比，用血清型b致敏的树突状细胞刺激的幼稚CD 4（+）T淋巴细胞引起更高水平的RANKL、RORC 2、TRAP（+）破骨细胞和骨吸收。RANKL与RORC 2呈正相关且共表达。牙周炎患者血清B型记忆性T淋巴细胞的检出率高于健康人。这些结果表明伴放线菌血清型B与RANKL的较高产生相关，并且这些水平的增加与Th 17淋巴细胞诱导、破骨细胞活化和骨吸收相关。
During periodontitis, alveolar bone resorption is associated with activation of T helper type 17 (Th17) lymphocytes and receptor activator of nuclear factor-κB ligand (RANKL) -induced osteoclasts. We previously reported that serotype b of Aggregatibacter actinomycetemcomitans has a higher capacity to trigger Th17-type differentiation and function in activated T lymphocytes and its lipopolysaccharide is a more potent immunogen compared with the other serotypes. This study aimed to investigate whether serotype b of A. actinomycetemcomitans induces higher Th17-associated RANKL production, RANKL-induced osteoclast activation, and antigen-specific memory T lymphocyte proliferation. On naive CD4(+) T lymphocytes stimulated with autologous dendritic cells primed with different A. actinomycetemcomitans serotypes, RANKL production, T-bet, GATA-3, RORC2 and Foxp3 expression, RORC2/RANKL intracellular double-expression, TRAP(+) osteoclast activation, and bone resorption were quantified. The frequency of proliferating memory T lymphocytes in response to A. actinomycetemcomitans serotypes was determined in periodontitis and healthy subjects. Naive CD4(+) T lymphocytes stimulated by serotype b-primed dendritic cells elicited higher levels of RANKL, RORC2, TRAP(+) osteoclasts, and bone resorption than the same cells stimulated with the other serotypes. RANKL positively correlated and co-expressed with RORC2. Memory T lymphocytes responding to serotype b were more frequently detected in periodontitis patients than healthy subjects. These results indicate that serotype b of A. actinomycetemcomitans is associated with higher production of RANKL and these increased levels are associated with Th17 lymphocyte induction, osteoclast activation, and bone resorption.
影响因子：
30.3
作者：
Jiao Y;Darzi Y;Tawaratsumida K;Marchesan JT;Hasegawa M;Moon H;Chen GY;Núñez G;Giannobile WV;Raes J;Inohara N
通讯作者：
Inohara N
影响因子：
15.9
作者：
Teng, YTA;Nguyen, H;Penninger, JM
通讯作者：
Penninger, JM
影响因子：
4.3
作者：
Jin, Qiming;Cirelli, Joni A.;Giannobile, William V.
通讯作者：
Giannobile, William V.