Experimental subarachnoid hemorrhage in rats: comparison of two endovascular perforation techniques with respect to success rate, confounding pathologies and early hippocampal tissue lesion pattern.
Experimental subarachnoid hemorrhage in rats: comparison of two endovascular perforation techniques with respect to success rate, confounding pathologies and early hippocampal tissue lesion pattern.
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大鼠实验性蛛网膜下腔出血：在成功率，混淆病理和早期海马组织病变模式方面的两种血管内穿孔技术的比较。
DOI：
10.1371/journal.pone.0123398
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Coburn M
中科院分区：
文献类型：
--
作者：
Höllig A;Weinandy A;Nolte K;Clusmann H;Rossaint R;Coburn M
最近，除了诱导实验性蛛网膜下腔出血（SAH）的“经典”血管内单股穿孔技术外，还描述了使用通过导管推进的钨丝的改良。我们的目的是评估两种技术的成功率（诱导SAH，无混杂病理）作为主要终点。此外，作为早期脑损伤证据的早期组织病变模式将作为次要终点进行分析。Sprague道利大鼠（n=39）被随机分配接受假手术（n=4）、使用“经典”技术的SAH（n=18）或使用改良技术的SAH（n=17）。分析颅内压（ICP）和局部脑血流量（rCBF）的过程;记录SAH后6或24 h的后续病理学。通过免疫组织化学和蛋白质印迹分析海马组织样品。无论混杂病理如何，SAH诱导与技术类型无关（p=0.679）。病死率无显著差异（经典：40%;改良：20%; p=0.213）。经典技术成功诱导SAH而无侧支ICH或SDH的可能性为40%，改良技术为86.7%（p=0.008）。两组之间的峰值ICP水平显著不同（经典：94 +/- 23 mmHg;改良：68 +/- 19 mmHg; p=0.003）。SAH后6小时早期细胞应激反应和凋亡途径活化的证据被证明。应激反应的程度与技术类型无关。两种测试技术都成功地产生SAH，包括海马组织中早期应激反应和凋亡途径的激活。然而，采用改良钨丝技术诱导SAH的混淆病理较少。
Recently aside from the “classic” endovascular monofilament perforation technique to induce experimental subarachnoid hemorrhage (SAH) a modification using a tungsten wire advanced through a guide tube has been described. We aim to assess both techniques for their success rate (induction of SAH without confounding pathologies) as primary endpoint. Further, the early tissue lesion pattern as evidence for early brain injury will be analyzed as secondary endpoint. Sprague Dawley rats (n=39) were randomly assigned to receive either Sham surgery (n=4), SAH using the “classic” technique (n=18) or using a modified technique (n=17). Course of intracranial pressure (ICP) and regional cerebral blood flow (rCBF) was analyzed; subsequent pathologies were documented either 6 or 24 h after SAH. Hippocampal tissue samples were analyzed via immunohistochemistry and western blotting. SAH-induction, regardless of confounding pathologies, was independent from type of technique (p=0.679). There was no significant difference concerning case fatality rate (classic: 40%; modified: 20%; p=0.213). Successful induction of SAH without collateral ICH or SDH was possible in 40% with the classic and in 86.7% with the modified technique (p=0.008). Peak ICP levels differed significantly between the two groups (classic: 94 +/- 23 mmHg; modified: 68 +/- 19 mmHg; p=0.003). Evidence of early cellular stress response and activation of apoptotic pathways 6 h after SAH was demonstrated. The extent of stress response is not dependent on type of technique. Both tested techniques successfully produce SAH including activation of an early stress response and apoptotic pathways in the hippocampal tissue. However, the induction of SAH with less confounding pathologies was more frequently achieved with the modified tungsten wire technique.
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影响因子：
2.5
作者：
Friedrich V;Flores R;Sehba FA
通讯作者：
Sehba FA
影响因子：
8.3
作者：
DELGADO, TJ;BRISMAR, J;SVENDGAARD, NA
通讯作者：
SVENDGAARD, NA
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11.2
作者：
O'Collins, VE;Macleod, MR;Howells, DW
通讯作者：
Howells, DW
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6.9
作者：
Greenhalgh, A. D.;Rothwell, N. J.;Allan, S. M.
通讯作者：
Allan, S. M.
影响因子：
4
作者：
Chaitanya, Ganta Vijay;Babu, Phanithi Prakash
通讯作者：
Babu, Phanithi Prakash