Diversity of Phage Types among Archived Cultures of the Demerec Collection of Salmonella enterica serovar Typhimurium Strains
Diversity of Phage Types among Archived Cultures of the Demerec Collection of Salmonella enterica serovar Typhimurium Strains
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肠沙门氏菌鼠伤寒菌株 Demerec 保藏培养物中噬菌体类型的多样性
DOI：
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发表时间：
2004
影响因子：
4.4
通讯作者：
A. Eisenstark
中科院分区：
文献类型：
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作者：
W. Rabsch;R. Allen Helm;A. Eisenstark
数千株肠道沙门氏菌血清型鼠伤寒杆菌LT2和LT7培养物最初由M. Demerec收集，并在琼脂刺瓶中密封了33至46年，为进化和突变研究提供了资源。其中74个小瓶的培养物是几十年前密封并储存在营养琼脂刺中的细胞的后代，通过Callow和Felix， Lilleengen和Anderson系统进行噬菌体分型。在53株LT2存档菌株中，有16株与未存档菌株具有相同的噬菌体类型。其他37个存档的培养物在噬菌体分型模式上与测序菌株不同。这37株菌株被分为10种不同的噬菌体类型。在19株LT7菌株中，只有1株噬菌体分型与亲本相似，18株不同。这18个菌株分为8种不同的噬菌体类型。开发分型系统是为了跟踪流行病从源头到消费者，以及地理传播。噬菌体分型的价值取决于在整个研究过程中任何给定菌株的噬菌体类型的稳定性。因此，在这些存档的文化中观察到的随时间的变化特别令人惊讶。这种惊人的多样性可能的机制包括：原噬菌体的丧失、重组事件导致的原噬菌体嵌合、细菌细胞表面噬菌体受体位点的变化或限制性修饰系统的突变。
ABSTRACT The existence of several thousand Salmonella enterica serovar Typhimurium LT2 and LT7 cultures originally collected by M. Demerec and sealed in agar stab vials for 33 to 46 years is a resource for evolutionary and mutational studies. Cultures from 74 of these vials, descendants of cells sealed and stored in nutrient agar stabs several decades ago, were phage typed by the Callow and Felix, Lilleengen, and Anderson systems. Among 53 LT2 archived strains, 16 had the same phage type as the nonarchival sequenced LT2 strain. The other 37 archived cultures differed in phage typing pattern from the sequenced strain. These 37 strains were divided into 10 different phage types. Among the 19 LT7 strains, only one was similar to the parent by phage typing, while 18 were different. These 18 strains fell into eight different phage types. The typing systems were developed to track epidemics from source to consumer, as well as geographic spread. The value of phage typing is dependent upon the stability of the phage type of any given strain throughout the course of the investigation. Thus, the variation over time observed in these archived cultures is particularly surprising. Possible mechanisms for such striking diversity may include loss of prophages, prophage mosaics as a result of recombination events, changes in phage receptor sites on the bacterial cell surface, or mutations in restriction-modification systems.
影响因子：
2.1
作者：
Edwards, K;Linetsky, I;Eisenstark, A
通讯作者：
Eisenstark, A