Imaging streptavidin 2D crystals on biotinylated lipid monolayers at high resolution with the atomic force microscope
Imaging streptavidin 2D crystals on biotinylated lipid monolayers at high resolution with the atomic force microscope
复制标题
使用原子力显微镜对生物素化脂质单层上的链霉亲和素二维晶体进行高分辨率成像
DOI：
10.1046/j.1365-2818.1999.00434.x
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发表时间：
1999
影响因子：
2
通讯作者：
A. Engel
中科院分区：
文献类型：
--
作者：
S. Scheuring;D. Müller;P. Ringler;J. Heymann;A. Engel
链霉亲和素晶体生长在生物素化的脂质单层在空气/水界面和转移到高度取向的热解石墨（HOPG）。使用原子力显微镜，这些阵列可以成像到低于1 nm的分辨率。将获得的表面形貌与负染电子显微镜图像和X射线晶体学确定的原子模型进行比较。链霉亲和素四聚体（60 kDa）将两个游离的生物素结合位点暴露于缓冲液，而两个位点被与脂质单层的连接占据。 因此，链霉亲和素2D晶体可用作结合生物素化化合物的纳米级基质。此外，这种基于HOPG的制备方法提供了一种通用的新方法来研究蛋白质阵列组装在脂质单分子膜上的结构与AFM。
Streptavidin crystals were grown on biotinylated lipid monolayers at an air/water interface and transferred onto highly oriented pyrolytic graphite (HOPG). These arrays could be imaged to a resolution below 1 nm using the atomic force microscope. The surface topographs obtained were compared with negative‐stain electron microscopy images and the atomic model as determined by X‐ray crystallography. The streptavidin tetramer (60 kDa) exposes two free biotin‐binding sites to the buffer solution, while two are occupied by linkage to the lipid monolayer. Therefore, the streptavidin 2D crystals can be used as nanoscale matrices for binding biotinylated compounds. Furthermore, this HOPG‐based preparation method provides a general novel approach to study the structure of protein arrays assembled on lipid monolayers with the AFM.
伴侣蛋白 GroEL 和 GroES：来自原子力显微镜的视图。
影响因子：
3.4
作者：
Mou,J;Sheng,S;Ho,R;Shao,Z
通讯作者：
Shao,Z
影响因子：
3.4
作者：
DARST, SA;AHLERS, M;KORNBERG, RD
通讯作者：
KORNBERG, RD
影响因子：
5.6
作者：
Czajkowsky, DM;Sheng, ST;Shao, ZF
通讯作者：
Shao, ZF