Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty.
Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty.
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伊氏锥虫的基因组和系统发育分析揭示了与布氏锥虫的广泛相似性以及运动障碍的多个独立起源
DOI：
10.1371/journal.pntd.0003404
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发表时间：
2015-01
影响因子：
3.8
通讯作者：
Schnaufer A
中科院分区：
文献类型：
--
作者：
Carnes J;Anupama A;Balmer O;Jackson A;Lewis M;Brown R;Cestari I;Desquesnes M;Gendrin C;Hertz-Fowler C;Imamura H;Ivens A;Kořený L;Lai DH;MacLeod A;McDermott SM;Merritt C;Monnerat S;Moon W;Myler P;Phan I;Ramasamy G;Sivam D;Lun ZR;Lukeš J;Stuart K;Schnaufer A
伊氏锥虫与T.布鲁氏菌组：不依赖于作为专性载体的采采蝇，并且不依赖于对功能性线粒体DNA（动质体或kDNA）的需要。为了更好地理解这些差异的分子原因和后果，我们对一个运动不能型T细胞的基因组进行了测序。evansi菌株的分离株，并与T. B.布氏参考菌株注释的T. evansi的基因组与参考序列的相似性为94.9%。B.在T. evansi，94.6%的非重复直向同源物具有95%或更高的核苷酸同一性。有趣的是，几个原环素相关基因（PAG）被破坏或没有发现在这个T。evansi菌株，表明在昆虫生命周期阶段不存在的情况下选择性丧失功能。令人惊讶的是，在T. evansi对所有978个预测代表T. brucei，虽然其中一小部分可能已经失去功能。与先前的结果一致，发现F1 FO-ATP合酶γ亚基具有A281缺失，其在不存在kDNA的情况下参与线粒体膜电位的产生。在T.埃文斯读着。系统发育分析表明，该菌株属于T. evansi菌株，也包括分类为T. Equiperdum。至少有三种其他类型的T。evansi或T.马鹿是独立出现的总的来说，T. evansi基因组序列显示T. brucei，支持T. evansi应归为T.布鲁塞。
Two key biological features distinguish Trypanosoma evansi from the T. brucei group: independence from the tsetse fly as obligatory vector, and independence from the need for functional mitochondrial DNA (kinetoplast or kDNA). In an effort to better understand the molecular causes and consequences of these differences, we sequenced the genome of an akinetoplastic T. evansi strain from China and compared it to the T. b. brucei reference strain. The annotated T. evansi genome shows extensive similarity to the reference, with 94.9% of the predicted T. b. brucei coding sequences (CDS) having an ortholog in T. evansi, and 94.6% of the non-repetitive orthologs having a nucleotide identity of 95% or greater. Interestingly, several procyclin-associated genes (PAGs) were disrupted or not found in this T. evansi strain, suggesting a selective loss of function in the absence of the insect life-cycle stage. Surprisingly, orthologous sequences were found in T. evansi for all 978 nuclear CDS predicted to represent the mitochondrial proteome in T. brucei, although a small number of these may have lost functionality. Consistent with previous results, the F1FO-ATP synthase γ subunit was found to have an A281 deletion, which is involved in generation of a mitochondrial membrane potential in the absence of kDNA. Candidates for CDS that are absent from the reference genome were identified in supplementary de novo assemblies of T. evansi reads. Phylogenetic analyses show that the sequenced strain belongs to a dominant group of clonal T. evansi strains with worldwide distribution that also includes isolates classified as T. equiperdum. At least three other types of T. evansi or T. equiperdum have emerged independently. Overall, the elucidation of the T. evansi genome sequence reveals extensive similarity of T. brucei and supports the contention that T. evansi should be classified as a subspecies of T. brucei.
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将统计测量数据导入 Artemis 可以增强利什曼原虫基因组计划中的基因识别。
影响因子：
3
作者：
Aggarwal G;Worthey EA;McDonagh PD;Myler PJ
通讯作者：
Myler PJ
影响因子：
3.4
作者：
Acestor, Nathalie;Panigrahi, Aswini K.;Ogata, Yuko;Anupama, Atashi;Stuart, Kenneth D.
通讯作者：
Stuart, Kenneth D.
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
DOI：
10.1073/pnas.1305404110
发表时间：
2013-09-03
影响因子：
11.1
作者：
Dean, Samuel;Gould, Matthew K.;Schnaufer, Achim C.
通讯作者：
Schnaufer, Achim C.
影响因子：
2.4
作者：
Claes, F;Agbo, EC;Büscher, P
通讯作者：
Büscher, P