Genome-wide DNA methylation changes associated with olfactory learning and memory in Apis mellifera.
Genome-wide DNA methylation changes associated with olfactory learning and memory in Apis mellifera.
复制标题
与蜜蜂嗅觉学习和记忆相关的全基因组 DNA 甲基化变化
DOI：
10.1038/s41598-017-17046-1
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发表时间：
2017-12-05
影响因子：
4.6
通讯作者：
Wang ZL
中科院分区：
文献类型：
--
作者：
Li Y;Zhang LZ;Yi Y;Hu WW;Guo YH;Zeng ZJ;Huang ZY;Wang ZL
蜜蜂是研究学习和记忆形成及其潜在分子机制的模式生物。虽然DNA甲基化在种姓分化中得到了很好的研究，但它在蜜蜂的学习和记忆中的作用尚不清楚。本研究采用全基因组亚硫酸盐测序（WGBS）方法分析了蜜蜂嗅觉学习和记忆过程中DNA甲基化的变化。共鉴定出853个显著差异甲基化区（DMRs）和963个差异甲基化基因（dmg）。我们发现，与未训练组相比，训练组648个基因中有440个DMRs高甲基化，336个基因中有274个DMRs低甲基化。在这些dmg中，许多是与学习和记忆有关的关键基因，如Creb、GABA
The honeybee is a model organism for studying learning and memory formation and its underlying molecular mechanisms. While DNA methylation is well studied in caste differentiation, its role in learning and memory is not clear in honeybees. Here, we analyzed genome-wide DNA methylation changes during olfactory learning and memory process in A. mellifera using whole genome bisulfite sequencing (WGBS) method. A total of 853 significantly differentially methylated regions (DMRs) and 963 differentially methylated genes (DMGs) were identified. We discovered that 440 DMRs of 648 genes were hypermethylated and 274 DMRs of 336 genes were hypomethylated in trained group compared to untrained group. Of these DMGs, many are critical genes involved in learning and memory, such as Creb, GABA
B
R and Ip3k, indicating extensive involvement of DNA methylation in honeybee olfactory learning and memory process. Furthermore, key enzymes for histone methylation, RNA editing and miRNA processing also showed methylation changes during this process, implying that DNA methylation can affect learning and memory of honeybees by regulating other epigenetic modification processes.
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影响因子：
11.4
作者：
Communi, D;Vanweyenberg, V;Erneux, C
通讯作者：
Erneux, C
DOI：
10.1073/pnas.1110184108
发表时间：
2011-11-08
影响因子：
11.1
作者：
Chen, Kaiyun;Richlitzki, Antje;Richmond, Janet E.
通讯作者：
Richmond, Janet E.
DOI：
10.1073/pnas.1202576109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Avargues-Weber, Aurore;Dyer, Adrian G.;Giurfa, Martin
通讯作者：
Giurfa, Martin
影响因子：
10.5
作者：
Ketting, RF;Fischer, SEJ;Plasterk, RHA
通讯作者：
Plasterk, RHA
影响因子：
3.6
作者：
Demares, Fabien;Drouard, Florian;Armengaud, Catherine
通讯作者：
Armengaud, Catherine