Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage.
Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage.
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DOI：
10.1126/sciadv.abj9670
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发表时间：
2022-05-06
期刊：
影响因子：
13.6
通讯作者：
Pogliano, Joe
中科院分区：
文献类型：
--
作者：
Chaikeeratisak, Vorrapon;Khanna, Kanika;Nguyen, Katrina T.;Egan, MacKennon E.;Enustun, Eray;Armbruster, Emily;Lee, Jina;Pogliano, Kit;Villa, Elizabeth;Pogliano, Joe
许多真核病毒在不同的亚细胞室中组装成熟的颗粒，但噬菌体通常被认为在宿主细胞细胞质中随机组装。在这里，我们展示了形成核的假单胞菌巨型噬菌体PhiPA3的病毒颗粒在细胞内组装成一种独特的结构，我们称之为噬菌体花束。我们发现，当衣壳在噬菌体核表面完成DNA包装后，尾巴组装并附着在衣壳上，这些颗粒随着时间的推移以球形模式积累，尾巴向内，头部向外，在特定的亚细胞位置形成花束。即使在错误定位的情况下，花束也会定位在与噬菌体细胞核相同的固定距离上，这表明有一种主动的定位机制。这些结果标志着在细菌内组织成熟病毒颗粒的途径的发现，并表明形成核的巨噬菌体像大多数真核病毒一样，在其裂解周期的所有阶段都是高度空间组织的。巨噬菌体在病毒颗粒组装和成熟过程中形成“噬菌体花束”，揭示了新的噬菌体组装途径。
Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm. Here, we show that viral particles of Pseudomonas nucleus-forming jumbo phage PhiPA3 assemble into a unique structure inside cells we term phage bouquets. We show that after capsids complete DNA packaging at the surface of the phage nucleus, tails assemble and attach to capsids, and these particles accumulate over time in a spherical pattern, with tails oriented inward and the heads outward to form bouquets at specific subcellular locations. Bouquets localize at the same fixed distance from the phage nucleus even when it is mispositioned, suggesting an active mechanism for positioning. These results mark the discovery of a pathway for organizing mature viral particles inside bacteria and demonstrate that nucleus-forming jumbo phages, like most eukaryotic viruses, are highly spatially organized during all stages of their lytic cycle. Jumbo phages form “phage bouquets” during viral particle assembly and maturation, revealing novel phage assembly pathway.
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