Activation of mouse peritoneal macrophages by synthetic glyceroglycolipid liposomes
Activation of mouse peritoneal macrophages by synthetic glyceroglycolipid liposomes
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合成甘油糖脂脂质体激活小鼠腹腔巨噬细胞
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Keizō Inoue
中科院分区：
文献类型：
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作者：
M. Naito;I. Kudo;Yukiko Mukai;S. Tsushima;H. Nomura;S. Nojima;Keizō Inoue
由化学合成的甘油糖脂如1，2-二棕榈酰-[β-纤维二糖基-（1′ → 3）]-甘油（Cel-DAG）、1，2-二棕榈酰-[β-乳糖基-（1′ → 3）]-甘油或1，2-二棕榈酰-[β-麦芽糖基-（1′ → 3）]-甘油组成的脂质体被发现增强ICR小鼠对可移植肿瘤细胞（肉瘤180）的保护性免疫。从用Cel-DAG在体内处理的小鼠制备的腹膜渗出液细胞在体外对小鼠白血病细胞系EL-4表现出细胞抑制活性。从该制剂中分离的贴壁细胞显示出类似的活性。注射多肽的小鼠的腹膜细胞在甘油糖脂脂质体存在下体外孵育时获得了明显的细胞抑制活性。单独的贴壁细胞组分显示出相当弱的细胞生长抑制活性时，预处理的甘油糖脂，并通过补充与非贴壁细胞组分恢复充分的活动。糖脂诱导杀肿瘤作用的能力受胆固醇含量的影响：随着胆固醇含量的增加，活性降低。不含胆固醇的糖脂脂质体比含胆固醇的脂质体更有效地被巨噬细胞摄取。胆固醇修饰甘油糖脂脂质体的表面性质。巨噬细胞的活化负责通过体内注射这些糖脂来增强针对肿瘤细胞的保护性免疫。
SummaryLiposomes composed of chemically synthesized glyceroglycolipids, such as 1,2-dipalmityl-[β-cellobiosyl-(1′ → 3)]-glycerol (Cel-DAG), 1,2-dipalmityl-[β-lactosyl-(1′ → 3)]-glycerol, or 1,2-dipalmityl-[β-maltosyl-(1′ → 3)]-glycerol, were found to enhance protective immunity against transplantable tumor cells (sarcoma 180) in ICR mice. Peritoneal exudate cells prepared from mice treated in vivo with Cel-DAG showed cytostatic activity in vitro against the mouse leukemia cell line, EL-4. Adherent cells separated from this preparation showed similar activity. Peritoneal cells from polypeptone-injected mice acquired appreciable cytostatic activity when incubated in vitro in the presence of glyceroglycolipid liposomes. The adherent cell fraction alone showed rather weak cytostatic activity when pretreated with the glyceroglycolipids, and full activity was restored by supplementing with the nonadherent cell fraction. The ability of glycolipids to induce tumoricidal effects was affected by cholesterol content: with increasing cholesterol content, the activities decreased. Cholesterol-free glycolipid liposomes were taken more efficiently by macrophages than cholesterol-containing liposomes. Cholersterol modifies the surface property of glyceroglycolipid liposomes. Activation of macrophages is responsible for enhancement of protective immunity against tumor cells by injection of these glycolipids in vivo.
兔肺泡巨噬细胞对甘露糖-牛血清白蛋白衍生物的结合和加工。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Hoppe,CA;Lee,YC
通讯作者：
Lee,YC
鉴定产生大量巨噬细胞激活因子的 T 细胞杂交瘤。
影响因子：
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作者：
Schreiber,RD;Altman,A;Katz,DH
通讯作者：
Katz,DH
巨噬细胞激活：杂交瘤MAF和聚(I)之间的协同作用。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Pidgeon,C;Schreiber,RD;Schultz,RM
通讯作者：
Schultz,RM