Association between Virulence Factors and TRAF1/4-1BB/Bcl-xL Expression in Gastric Mucosa Infected with Helicobacter pylori.
Association between Virulence Factors and TRAF1/4-1BB/Bcl-xL Expression in Gastric Mucosa Infected with Helicobacter pylori.
复制标题
DOI：
10.1155/2015/648479
复制
发表时间：
2015
影响因子：
2
通讯作者：
Yuan Y
中科院分区：
文献类型：
--
作者：
Wang F;Wu X;Liu Z;Bu G;Li X;Qu N;Peng J;Xu C;Shen S;Yuan Y
客观的。 CagA+/vacAs1+/vacAm1+ 幽门螺杆菌上调人胃上皮细胞中肿瘤坏死因子受体相关因子 1 (TRAF1)、肿瘤坏死因子受体超家族成员 9 (4-1BB) 和特大 B 细胞淋巴瘤 (Bcl-xL) 的表达。我们研究了胃病患者胃粘膜组织中cagA/vacAs1/vacAm1与TRAF1/4-1BB/Bcl-xL表达的相关性。方法。我们收集了 35 名慢性非萎缩性胃炎 (CG) 患者、41 名萎缩性胃炎患者、44 名肠化伴不典型增生 (IM) 患者和 28 名胃癌 (Ca) 患者的胃粘膜样本。使用蛋白质印迹法测定TRAF1、4-1BB和Bcl-xL的表达。采用聚合酶链式反应检测幽门螺杆菌中 cagA、vacAs1 和 vacAm1 的表达。结果。 IM和Ca患者中TRAF1、4-1BB和Bcl-xL的表达显着上调（与CG相比，P < 0.05）。 TRAF1、4-1BB、Bcl-xL表达升高的样本中，cagA+/vacAs1+/vacAm1+幽门螺杆菌感染病例较多（P < 0.05）。此外，在cagA+/vacAs1+/vacAm1+幽门螺杆菌感染病例中，TRAF1/4-1BB/Bcl-xL表达上调的样本数量非常多（44例，67.7%；P < 0.05）。结论。 IM和Ca的发病机制可能是由cagA+/vacAs1+/vacAm1+幽门螺杆菌促进的，可能是通过胃粘膜组织中TRAF1、4-1BB和Bcl-xL的上调来促进的。
Objective. CagA+/vacAs1+/vacAm1+ Helicobacter pylori upregulates the expression of tumor necrosis factor receptor–associated factor 1 (TRAF1), tumor necrosis factor receptor superfamily member 9 (4-1BB), and B-cell lymphoma-extra large (Bcl-xL) in human gastric epithelial cells. We investigated the correlation between cagA/vacAs1/vacAm1 and TRAF1/4-1BB/Bcl-xL expression in gastric mucosal tissue of patients with gastric disorders. Methods. We collected gastric mucosa samples from 35 chronic, nonatrophic gastritis (CG) patients, 41 atrophic gastritis patients, 44 intestinal metaplasia with atypical hyperplasia (IM) patients, and 28 gastric carcinoma (Ca) patients. The expression of TRAF1, 4-1BB, and Bcl-xL was determined using western blotting. The expression of cagA, vacAs1, and vacAm1 in H. pylori was examined with polymerase chain reaction. Results. The expression of TRAF1, 4-1BB, and Bcl-xL was significantly upregulated in IM and Ca patients (P < 0.05 compared with CG). There were more cases of cagA+/vacAs1+/vacAm1+ H. pylori infection in samples with elevated TRAF1, 4-1BB, or Bcl-xL expression (P < 0.05). Additionally, there were a remarkably large number of samples with upregulated TRAF1/4-1BB/Bcl-xL expression in cases of cagA+/vacAs1+/vacAm1+ H. pylori infection (44 cases, 67.7%; P < 0.05). Conclusions. The pathogenesis of IM and Ca may be promoted by cagA+/vacAs1+/vacAm1+ H. pylori, possibly via upregulated TRAF1, 4-1BB, and Bcl-xL in gastric mucosal tissue.
登录
查看更多内容
影响因子：
6.3
作者：
Satomi, Satoko;Yamakawa, Akiyo;Azuma, Takeshi
通讯作者：
Azuma, Takeshi
影响因子：
3.1
作者：
Matsumoto, Ayako;Isomoto, Hajime;Kohno, Shigeru
通讯作者：
Kohno, Shigeru
影响因子：
4.3
作者：
Ki, Mi-Ran;Hwang, Meeyul;Jeong, Kyu-Shik
通讯作者：
Jeong, Kyu-Shik
影响因子：
5
作者：
Rocco, Alba;Compare, Debora;Nardone, Gerardo
通讯作者：
Nardone, Gerardo
影响因子：
1.6
作者：
Yang, Liu;Levi, Edi;Majumdar, Adhip P N
通讯作者：
Majumdar, Adhip P N