High-throughput determination of RNA structures.

High-throughput determination of RNA structures.
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DOI:
10.1038/s41576-018-0034-x
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发表时间:
2018-10
期刊:
Nature reviews. Genetics
影响因子:
--
通讯作者:
Lucks JB
Lucks JB
中科院分区:
其他
文献类型:
--
作者:
Strobel EJ;Yu AM;Lucks JB

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RNA在执行和调节基本生命过程中的广泛作用正在迅速被发现。这引发了关于RNA功能如何通过RNA结构在复杂的细胞环境中协调过程的深刻问题。这些问题正在通过使用大规模并行测序以前所未有的通量询问RNA结构的技术来回答。在本文中,我们将越来越多的这些技术放在一个共同的概念框架上,回顾它们的技术基础,讨论它们数据的意义和用途,并强调它们在RNA生物学新领域的应用。
The broad roles of RNA in performing and regulating fundamental life processes are being uncovered at a rapid pace. This has sparked profound questions about how RNA function is enacted through RNA structures that orchestrate processes in the complex cellular environment. These questions are being answered with technologies that use massively parallel sequencing to interrogate RNA structure at unprecedented throughput. In this article, we place the growing number of these technologies on a common conceptual framework, review their technical underpinnings, discuss the meaning and use of their data, and highlight their application to new areas of RNA biology.
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