Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum
Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum
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随着红藻紫球藻生长辐照度的增加，PS I 天线复合体中的多肽减少
DOI：
10.1007/bf00032230
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发表时间：
1995
影响因子：
3.7
通讯作者：
E. Gantt
中科院分区：
文献类型：
--
作者：
Shi Tan;G. Wolfe;F. Cunningham;E. Gantt
从红色紫球藻细胞中分离的类囊体显示出基于叶绿素的PS I活性随着生长辐照度的增加而增加，即使在这样的细胞中光系统I和II的化学计量显示出很小的变化（Cunningham等人（1989）Plant Physiol 91：1179-1187）。PS I活性在280 μmol photons · m−2 · s−1（VHL）下驯化的细胞的类囊体中比在10 μmol photons · m−2 · s−1（LL）下驯化的细胞高26%，表明PS I的光吸收能力发生了变化。从VHL细胞中分离出PS I全复合物后发现，它们含有132±9个Chl/P700，而从LL细胞中获得的PS I全复合物含有165±4个Chl/P700。在SDS-PAGE上对PS I全复合物的多肽组成的检查表明，相对于LL复合物，VHL复合物中的三种多肽（19.5、21.0和22 kDa）显著减少。这些多肽属于最近在红藻中发现的新的LHC I复合物（Wolfe等（1994 a）Nature 367：566-568），其缺乏Chl B并包括至少六种不同的多肽。我们认为，PS I叶绿素天线的大小随着辐照度的增加而减少，这是由于LHC I天线复合体发生的变化。目前还缺乏与PS II核心复合物相关的Chl结合天线复合物的证据。在功能活性PS II制备物中未观察到LHC II型多肽（Wolfe等（1994 b）Biochimica Bioproteica Acta 1188：357-366），我们也未检测到与LHC II特异性抗血清（对衣原体和眼虫LHC II制备）显示免疫交叉反应性的多肽。
Thylakoids isolated from cells of the red alga Porphyridium cruentum exhibit an increased PS I activity on a chlorophyll basis with increasing growth irradiance, even though the stoichiometry of Photosystems I and II in such cells shows little change (Cunningham et al. (1989) Plant Physiol 91: 1179–1187). PS I activity was 26% greater in thylakoids of cells acclimated at 280 μmol photons · m−2 · s−1 (VHL) than in cells acclimated at 10 μmol photons · m−2 · s−1 (LL), indicating a change in the light absorbance capacity of PS I. Upon isolating PS I holocomplexes from VHL cells it was found that they contained 132±9 Chl/P700 while those obtained from LL cells had 165±4 Chl/P700. Examination of the polypeptide composition of PS I holocomplexes on SDS-PAGE showed a notable decrease of three polypeptides (19.5, 21.0 and 22 kDa) in VHL-complexes relative to LL-complexes. These polypeptides belong to a novel LHC I complex, recently discovered in red algae (Wolfe et al. (1994a) Nature 367: 566–568), that lacks Chl b and includes at least six different polypeptides. We suggest that the decrease in PS I Chl antenna size observed with increasing irradiance is attributable to changes occurring in the LHC I-antenna complex. Evidence for a Chl-binding antenna complex associated with PS II core complexes is lacking at this point. LHC II-type polypeptides were not observed in functionally active PS II preparations (Wolfe et al. (1994b) Biochimica Biophysica Acta 1188: 357–366), nor did we detect polypeptides that showed immunocross-reactivity with LHC II specific antisera (made to Chlamydomonas and Euglena LHC II).
将十二烷基硫酸钠-聚丙烯酰胺凝胶中的蛋白质定量电转移到带正电的尼龙膜上。
影响因子：
2.9
作者：
Peluso,RW;Rosenberg,GH
通讯作者：
Rosenberg,GH