Effects of cyanobacteria Oscillatoria sp. lipopolysaccharide on B cell activation and Toll-like receptor 4 signaling.
Effects of cyanobacteria Oscillatoria sp. lipopolysaccharide on B cell activation and Toll-like receptor 4 signaling.
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DOI：
10.1016/j.toxlet.2017.05.013
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发表时间：
2017-06-05
影响因子：
3.5
通讯作者：
Mayer AMS
中科院分区：
文献类型：
--
作者：
Swanson-Mungerson M;Incrocci R;Subramaniam V;Williams P;Hall ML;Mayer AMS
蓝细菌（“蓝绿藻”），如颤藻属，是在世界各地的淡水系统中发现的一组普遍存在的细菌，它们与人类和动物的疾病有关，在某些情况下甚至导致死亡。通过摄入受污染的水或食品，可接触蓝细菌。肠道暴露于这些细菌也会将它们的毒素，如脂多糖（LPS）暴露于肠道相关淋巴组织中的B细胞。然而，颤藻属LPS对B细胞活化的影响尚不清楚。为了检验颤藻属LPS暴露于鼠B细胞将导致B细胞活化的假设，将鼠B细胞在不存在或存在颤藻属LPS或E. coli LPS作为阳性对照。数据表明颤藻属LPS诱导B细胞增殖，上调MHC II和CD 86，增强抗原摄取并诱导低水平的IgM产生。另外的研究表明，这种低水平的刺激可能是由于颤藻属LPS诱导的不完全的TLR 4信号传导，因为在用颤藻属LPS刺激后，IRF-3在B细胞中不被诱导。这些发现对蓝藻对人类和动物的毒性机制具有重要意义。
Cyanobacteria (“blue-green algae”), such as Oscillatoria sp., are a ubiquitous group of bacteria found in freshwater systems worldwide that are linked to illness and in some cases, death among humans and animals. Exposure to cyanobacteria occurs via ingestion of contaminated water or food-products. Exposure of the gut to these bacteria also exposes their toxins, such as lipopolysaccharide (LPS), to B cells in the gut associated lymphoid tissue. However, the effect of Oscillatoria sp. LPS on B cell activation is unknown. To test the hypothesis that Oscillatoria sp. LPS exposure to murine B cells would result in B cell activation, murine B cells were incubated in the absence or presence of Oscillatoria sp. LPS or E. coli LPS as a positive control. The data indicate that Oscillatoria sp. LPS induces B cells to proliferate, upregulate MHC II and CD86, enhance antigen uptake and induce IgM production at low levels. Additional studies demonstrate that this low level of stimulation may be due to incomplete TLR4 signaling induced by Oscillatoria sp. LPS, since IRF-3 is not induced in B cells after stimulation with Oscillatoria sp. LPS. These findings have important implications for the mechanisms of toxicity of cyanobacteria in both humans and animals.
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影响因子：
5.7
作者：
De Paola, Massimiliano;Mariani, Alessandro;Fanelli, Roberto
通讯作者：
Fanelli, Roberto
LPS 在体内刺激 IgM 产生，无需非 B 细胞的帮助
影响因子：
3.2
作者：
Lu，Mingfang;Munford，Robert
通讯作者：
Munford，Robert
影响因子：
9.8
作者：
Lang-Yona, Naama;Lehahn, Yoav;Rudich, Yinon
通讯作者：
Rudich, Yinon
影响因子：
3.1
作者：
Jemmett, Kim;Macagno, Annalisa;Christodoulides, Myron
通讯作者：
Christodoulides, Myron
影响因子：
5.4
作者：
Minguet, Susana;Dopfer, Elaine P.;Schamel, Wolfgang W.
通讯作者：
Schamel, Wolfgang W.