Optimising storage conditions and processing of sheep urine for nitrogen cycle and gaseous emission measurements from urine patches.
Optimising storage conditions and processing of sheep urine for nitrogen cycle and gaseous emission measurements from urine patches.
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DOI：
10.1038/s41598-021-91498-4
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发表时间：
2021-06-09
影响因子：
4.6
通讯作者：
Cárdenas LM
中科院分区：
文献类型：
--
作者：
Charteris AF;Marsden KA;Evans JR;Barrat HA;Loick N;Jones DL;Chadwick DR;Cárdenas LM
在放牧系统中，牲畜尿液沉积斑块是养分循环的热点，也是氧化亚氮（N2O）排放的最重要来源。对尿液沉积影响的研究，包括例如确定国家特定的N2O排放系数，需要在实验中使用天然尿液，并面临获得相同成分但浓度不同的尿液的挑战。然而，很少有研究探讨了反刍动物尿液的储存条件和处理的重要性，用于随后的气体排放实验。我们进行了三个实验，羊尿，以确定最佳的储存条件和部分冷冻干燥是否可以用来浓缩尿液，同时保持成分的配置文件和随后的尿源性气体排放响应，一旦应用到土壤中。我们得出结论，在储存前过滤尿液，并在-20 °C下储存最能保持羊尿样品的含氮成分谱。此外，根据本研究中确定的14种尿液化学成分，将羊尿部分冻干成浓缩物是一种适当的方法，可将成分分布保持在较高的总浓度，并且不会改变羊尿来源的土壤气体排放。
In grazing systems, urine patches deposited by livestock are hotspots of nutrient cycling and the most important source of nitrous oxide (N2O) emissions. Studies of the effects of urine deposition, including, for example, the determination of country-specific N2O emission factors, require natural urine for use in experiments and face challenges obtaining urine of the same composition, but of differing concentrations. Yet, few studies have explored the importance of storage conditions and processing of ruminant urine for use in subsequent gaseous emission experiments. We conducted three experiments with sheep urine to determine optimal storage conditions and whether partial freeze-drying could be used to concentrate the urine, while maintaining the constituent profile and the subsequent urine-derived gaseous emission response once applied to soil. We concluded that filtering of urine prior to storage, and storage at − 20 °C best maintains the nitrogen-containing constituent profile of sheep urine samples. In addition, based on the 14 urine chemical components determined in this study, partial lyophilisation of sheep urine to a concentrate represents a suitable approach to maintain the constituent profile at a higher overall concentration and does not alter sheep urine-derived soil gaseous emissions.
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