Alterations of plant architecture and phase transition by the phytoplasma virulence factor SAP11.
Alterations of plant architecture and phase transition by the phytoplasma virulence factor SAP11.
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DOI：
10.1093/jxb/ery318
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发表时间：
2018-11-26
影响因子：
6.9
通讯作者：
Yang JY
中科院分区：
文献类型：
--
作者：
Chang SH;Tan CM;Wu CT;Lin TH;Jiang SY;Liu RC;Tsai MC;Su LW;Yang JY
植原体SAP11效应子在控制腋生分生组织发育的关键因子TB/CyC-TCPs中获得了不稳定的基本活性，并且是导致丛枝病症状的核心毒力因子。作为连接植物免疫和发育过程的关键调节因子，TcP(Teosinte-Branked，环虫纲，增殖因子1和2)转录因子越来越多地被证明是致病效应分子的靶标。我们在这里报道了TB/CyC(Teosinte-Branked/CycloidEA)-TCP被植原体SAP11效应子破坏了稳定性，导致腋生分生组织的增殖。虽然从进化上不同的植原体簇中鉴定出的SAP11效应器具有高度的序列多样性，但这些效应器获得了破坏TB/CyC-TCPs稳定的基本活性。此外，我们还证明了miR156/SPLs和miR172/AP2模块，这两个模块代表了参与植物相变的关键调控中心，受到了植物黄化植原体菌株Witches‘Broom(AY-WB)蛋白SAP11的调控。在转SAP11AYWB基因的拟南芥植株中，观察到与开花相关的基因表达发生显著变化的晚花表型。这些形态和分子变化与SAP11效应器破坏CIN(CINCINNAA)-TCPs的能力有关。虽然并不是所有推测的SAP11效应子在调节寄主植物的形态和生理变化方面都表现出广谱的活性，但它们是导致植原体引起的丛枝病症状的核心毒力因子。
Phytoplasma SAP11 effectors acquired fundamental activity in destabilizing TB/CYC-TCPs, the key factors controlling axillary meristem development, and serve as core virulence factors responsible for the witches’ broom symptom. As key mediators linking developmental processes with plant immunity, TCP (TEOSINTE-BRANCHED, CYCLOIDEA, PROLIFERATION FACTOR 1 and 2) transcription factors have been increasingly shown to be targets of pathogenic effectors. We report here that TB/CYC (TEOSINTE-BRANCHED/CYCLOIDEA)-TCPs are destabilized by phytoplasma SAP11 effectors, leading to the proliferation of axillary meristems. Although a high degree of sequence diversity was observed among putative SAP11 effectors identified from evolutionarily distinct clusters of phytoplasmas, these effectors acquired fundamental activity in destabilizing TB/CYC-TCPs. In addition, we demonstrate that miR156/SPLs and miR172/AP2 modules, which represent key regulatory hubs involved in plant phase transition, were modulated by Aster Yellows phytoplasma strain Witches’ Broom (AY-WB) protein SAP11. A late-flowering phenotype with significant changes in the expression of flowering-related genes was observed in transgenic Arabidopsis plants expressing SAP11AYWB. These morphological and molecular alterations were correlated with the ability of SAP11 effectors to destabilize CIN (CINCINNATA)-TCPs. Although not all putative SAP11 effectors display broad-spectrum activities in modulating morphological and physiological changes in host plants, they serve as core virulence factors responsible for the witches’ broom symptom caused by phytoplasmas.
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影响因子：
4.5
作者：
Kubota A;Ito S;Shim JS;Johnson RS;Song YH;Breton G;Goralogia GS;Kwon MS;Laboy Cintrón D;Koyama T;Ohme-Takagi M;Pruneda-Paz JL;Kay SA;MacCoss MJ;Imaizumi T
通讯作者：
Imaizumi T
影响因子：
4.5
作者：
Liu Y;Walavalkar NM;Dozmorov MG;Rich SS;Civelek M;Guertin MJ
通讯作者：
Guertin MJ
影响因子：
9.8
作者：
MacLean AM;Orlovskis Z;Kowitwanich K;Zdziarska AM;Angenent GC;Immink RG;Hogenhout SA
通讯作者：
Hogenhout SA
影响因子：
7.4
作者：
Lu, Yen-Ting;Li, Meng-Ying;Yang, Jun-Yi
通讯作者：
Yang, Jun-Yi
影响因子：
11.6
作者：
Aguilar-Martinez, Jose Antonio;Poza-Carrion, Cesar;Cubas, Pilar
通讯作者：
Cubas, Pilar