Thrombopoietic stimulating activity of rhTyrRS (Y341A).
Thrombopoietic stimulating activity of rhTyrRS (Y341A).
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rhTyrRS (Y341A) 的血小板生成刺激活性
DOI：
10.1038/s41598-017-12073-4
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发表时间：
2017-09-22
影响因子：
4.6
通讯作者：
Wei M
中科院分区：
文献类型：
--
作者：
Jinchao Y;Yanling Z;Xu W;Bing Z;Yuhao Y;Weiran Z;Shaoyang S;Liyun M;Yun S;Ling Z;Min Y;Wei M
Tumor radiotherapy induces hematopoietic organ damage and reduces thrombocyte counts. Thrombocytopenia is a common disease. Some studies have shown that tRNA synthetase plays not only catalytic tRNA aminoacylation roles, but also functions similarly to cytokines. Recombinant human tyrosyl-tRNA synthetase with a mutated Y341A (rhTyrRS (Y341A)) promotes megakaryocyte migrate from bone marrow to peripheral blood. It would promote megakaryocytes in the lungs adhering to vascular endothelial cells and resulting in the platelet production. The purpose of this research was to investigate the efficacy of rhTyrRS (Y341A) as a therapy for thrombocytopenia and to explore its mechanism of action. We found platelet number was effectively increased by rhTyrRS (Y341A) via platelet count and reticulated platelets (RPs) flow cytometry. We also demonstrated radiation-induced thrombocytopenia could be prevented by rhTyrRS (Y341A). The results of immunohistochemistry and H&E staining showed the number of pulmonary mature megakaryocytes was significantly increased in rhTyrRS (Y341A) treated groups. In transgenic zebrafish larvae, confocal microscopy results showed rhTyrRS (Y341A) promoted the migration and adhesion of megakaryocytes. These results suggested that rhTyrRS (Y341A) promote megakaryocytes in bone marrow migrating to lungs through blood circulation. rhTyrRS (Y341A) may be an effective medicine which could be used to treat patients suffering from thrombocytopenia.
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影响因子：
13.8
作者：
Schimmel, P;De Pouplana, LR
通讯作者：
De Pouplana, LR
DOI：
10.1073/pnas.012602099
发表时间：
2002-01-08
影响因子：
11.1
作者：
Wakasugi, K;Slike, BM;Schimmel, P
通讯作者：
Schimmel, P
DOI：
10.1016/s0167-4781(97)00233-9
发表时间：
1998-04-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Liang, D;Xu, XZ;Ge, RW
通讯作者：
Ge, RW
影响因子：
20.3
作者：
Lin, HF;Traver, D;Handin, RI
通讯作者：
Handin, RI
影响因子：
--
作者：
Kapoor M;Otero FJ;Slike BM;Ewalt KL;Yang XL
通讯作者：
Yang XL