Hyaluronan‐independent lodgment of CD44+ lymphoma cells in lymphoid organs
Hyaluronan‐independent lodgment of CD44+ lymphoma cells in lymphoid organs
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CD44+淋巴瘤细胞在淋巴器官中的透明质酸独立沉积
DOI：
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发表时间：
1997
影响因子：
6.4
通讯作者：
D. Naor
中科院分区：
文献类型：
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作者：
Ronit Vogt Sionov and;D. Naor
我们之前发现针对 CD44 分子恒定区的单克隆抗体 (MAb) 可阻断小鼠 LB T 细胞淋巴瘤的淋巴结浸润，表明这种糖蛋白在 LB 细胞传播过程中发挥作用。在本研究中，我们研究了局部肿瘤中的LB细胞是否必须经历CD44表型的改变才能迁移到并侵入远端淋巴结，以及激活CD44的主要配体透明质酸（HA）是否在此过程中充当介质。我们比较了非 HA 结合剂 LB 细胞系与组成型 HA 结合剂 HA9 亚系的体内行为。我们的结果表明，淋巴器官浸润的 LB 细胞表达的泛 CD44 以及含有 V4 和 V6 的 CD44 变体水平与局部生长和培养的 LB 细胞中的相应细胞相似。所测试的HA9细胞的CD44表型在转移过程中也保持不变。即使在淋巴结浸润后，LB细胞仍然无法结合HA，而HA9细胞保留了有效的HA结合能力。组成型 HA 结合剂 HA9 细胞表达的泛 CD44 水平比亲本 LB 细胞高约 10 倍，并且 V4 和 V6 外显子产物水平升高，形成局部肿瘤并侵入淋巴结和脾脏，与亲本 LB 细胞一样，尽管速度慢得多。我们的研究结果表明，细胞表面表达的 CD44 量、HA 结合能力和致瘤性之间没有直接相关性。此外，与HA的相互作用对于LB细胞在淋巴器官中的定位来说并不是必需的，并且如在HA9细胞中观察到的紧密的细胞-HA相互作用，虽然可能会延缓肿瘤的扩散，但并不能阻止肿瘤细胞的扩散。国际。 J. Cancer 71:462-469, 1997。© 1997 Wiley-Liss Inc.
We previously found that monoclonal antibodies (MAbs) directed against the constant region of the CD44 molecule block lymph node infiltration of a mouse LB T‐cell lymphoma, suggesting a role for this glycoprotein in the LB cell dissemination process. In the present study, we investigated whether LB cells in the local tumor must undergo a change in the CD44 phenotype to be able to migrate to and invade the remote lymph nodes, and if hyaluronic acid (HA), the principal ligand of activated CD44, functions as a mediator in this process. We compared the in vivo behavior of a non‐HA‐binder LB cell line with that of a constitutive HA‐binder HA9 subline. Our results show that the lymphoid organ‐infiltrating LB cells express similar levels of pan‐CD44 and V4‐ and V6‐containing CD44 variants, as the corresponding cells in the local growth and the cultured LB cells. The tested CD44 phenotype of HA9 cells also remained unchanged during the metastatic process. Even after lymph node infiltration, LB cells remained incapable of binding HA, whereas the HA9 cells retained an efficient HA‐binding capacity. The constitutive HA‐binder HA9 cells that expressed an approximately 10‐fold higher level of pan‐CD44 than did the parental LB cells, as well as an elevated level of the V4 and V6 exon products formed a local tumor and invaded both lymph nodes and spleen, as did the parental LB cells, albeit at a much slower rate. Our finding indicates that there is no direct correlation between the amount of CD44 expressed on the cell surface, the HA‐binding capacity and tumorigenicity. Moreover, interaction with HA is not obligatory for LB cell localization in the lymphoid organs, and a tight cell‐HA interaction, as observed in HA9 cells, does not prevent tumor cell dissemination, although it may retard tumor spread. Int. J. Cancer 71:462‐469, 1997. © 1997 Wiley‐Liss Inc.
影响因子：
11.2
作者：
Y. Guo;J. Ma;J. Wang;X. Che;J. Narula;M. Bigby;M. Wu;M. Sy
通讯作者：
Y. Guo;J. Ma;J. Wang;X. Che;J. Narula;M. Bigby;M. Wu;M. Sy
影响因子：
11.2
作者：
Lurong Zhang;C. Underhill;Lieping Chen
通讯作者：
Lurong Zhang;C. Underhill;Lieping Chen