ROP INTERACTIVE PARTNER b interacts with the ROP GTPase RACB and supports fungal penetration into barley epidermal cells
ROP INTERACTIVE PARTNER b interacts with the ROP GTPase RACB and supports fungal penetration into barley epidermal cells
复制标题
ROP INTERACTIVE PARTNER b 与 ROP GTPase RACB 相互作用并支持真菌渗透到大麦表皮细胞
DOI：
10.1101/750265
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Hückelhoven R
中科院分区：
文献类型：
--
作者：
McCollum C;Engelhardt S;Hückelhoven R
植物Rho蛋白（ROP）G蛋白是植物发育过程中细胞极化过程的重要组成部分。大麦（Hordeum vulgare）ROP蛋白RACB是大麦与大麦白粉病真菌Blumeria graminisf相互作用的易感因子。大麦锈病菌（Bgh）。RACB还驱动极性细胞的发育，这一功能可能在大麦表皮细胞中真菌吸器的形成过程中发挥作用。为了了解大麦与Bgh相互作用过程中的RACB信号，我们寻找了RACB的潜在下游相互作用物。在这里，我们表明ROP相互作用伴侣B（RIP B;同义词：组成性活性ROP B的相互作用物）直接与酵母和植物中的RACB相互作用。RIPb的过表达支持大麦对Bgh的易感性。RIPb在微管处与自身进一步相互作用。然而，与活化的RACB的相互作用主要发生在质膜上。RIPb和RACB都被招募到发育中吸器颈部周围的真菌攻击位点，表明局部增强的ROP活性。我们进一步为RIPb蛋白的不同结构域分配了不同的功能。N-末端卷曲螺旋CC 1结构域是微管定位所需的，而C-末端卷曲螺旋CC 2结构域足以与RACB相互作用并在质膜上实现易感性功能。因此，RIPb似乎定位于微管，然后被激活的RACB招募，在吸器复合体形成期间在质膜上发挥功能。
Rho of Plants (ROP) G-proteins are key components of cell polarization processes in plant development. The barley (Hordeum vulgare) ROP protein RACB is a susceptibility factor in the interaction of barley with the barley powdery mildew fungusBlumeria graminisf. sp.hordei(Bgh). RACB also drives polar cell development, and this function might be coopted during the formation of fungal haustoria in barley epidermal cells. To understand RACB signaling during the interaction of barley withBgh, we searched for potential downstream interactors of RACB. Here, we show that ROP INTERACTIVE PARTNER b (RIPb; synonym: INTERACTOR OF CONSTITUTIVE ACTIVE ROP b) directly interacts with RACB in yeast and in planta. Overexpression of RIPb supports the susceptibility of barley toBgh. RIPb further interacts with itself at microtubules. However, the interaction with activated RACB largely takes place at the plasma membrane. Both RIPb and RACB are recruited to the site of fungal attack around the neck of developing haustoria, suggesting locally enhanced ROP activity. We further assigned different functions to different domains of the RIPb protein. The N-terminal coiled-coil CC1 domain is required for microtubule localization, while the C-terminal coiled-coil CC2 domain is sufficient to interact with RACB and to fulfill a function in susceptibility at the plasma membrane. Hence, RIPb appears to be localized at microtubules and is then recruited by activated RACB for a function at the plasma membrane during formation of the haustorial complex.
登录
查看更多内容
DOI：
10.1073/pnas.98.2.759
发表时间：
2001-01
影响因子：
11.1
作者：
Eiichiro Ono;H. Wong;T. Kawasaki;M. Hasegawa;O. Kodama;K. Shimamoto
通讯作者：
Eiichiro Ono;H. Wong;T. Kawasaki;M. Hasegawa;O. Kodama;K. Shimamoto
影响因子：
7.4
作者：
Schultheiss, H;Dechert, C;Hückelhoven, R
通讯作者：
Hückelhoven, R
DOI：
10.1186/1939-8433-6-4
发表时间：
2013-02-06
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Kawahara Y;de la Bastide M;Hamilton JP;Kanamori H;McCombie WR;Ouyang S;Schwartz DC;Tanaka T;Wu J;Zhou S;Childs KL;Davidson RM;Lin H;Quesada-Ocampo L;Vaillancourt B;Sakai H;Lee SS;Kim J;Numa H;Itoh T;Buell CR;Matsumoto T
通讯作者：
Matsumoto T
影响因子：
27.5
作者：
Li, Shundai;Gu, Ying;Yang, Zhen-Biao
通讯作者：
Yang, Zhen-Biao
影响因子：
5.3
作者：
Hoefle, Caroline;McCollum, Christopher;Hueckelhoven, Ralph
通讯作者：
Hueckelhoven, Ralph