ZIKV infection differentially affects the transcriptional profiles in HTR8 and U251 cells.
ZIKV infection differentially affects the transcriptional profiles in HTR8 and U251 cells.
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DOI：
10.1016/j.virusres.2023.199166
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发表时间：
2023-09
期刊：
影响因子：
5
通讯作者：
Luo, Huanle
中科院分区：
文献类型：
--
作者：
Chen, Qiqi;Li, Nina;Zeng, Shike;Wu, Shu;Luo, Xin;Zhang, Shengze;Zhu, Lin;Wu, Jiani;Xie, Ting;Bai, Shaohui;Zhang, Hao;Jiang, Zhiyuan;Lin, Shaoli;Wu, Nan;Jiang, Ying;Fang, Shisong;Wang, Xin;Shu, Yuelong;Luo, Huanle
关键词：
ZIKV对胎盘滋养层细胞和人脑细胞具有不同的感染模式。ZIKV感染诱导干扰素、炎性细胞因子和趋化因子产生。TNF-α中和可促进HTR 8和U251细胞之间的ZIKV感染。寨卡病毒（ZIKV）导致孕妇严重出生缺陷的机制尚不清楚。胎盘和大脑中的细胞嗜性在ZIKV发病机制中起着至关重要的作用，导致先天性Zika综合征（CZS）。为了鉴定参与ZIKV感染的宿主因子，我们比较了ZIKV感染的人早期妊娠胎盘滋养层细胞HTR 8/SVneo和人胶质母细胞瘤星形细胞瘤细胞系U251的转录谱。我们的结果表明，ZIKV在HTR 8中表现出比在U251细胞中更低的mRNA复制和蛋白质表达速率，同时显示出更高的感染性病毒颗粒释放。然而，在ZIKV感染的U251细胞中发现的差异表达基因（DEG）的数量多于ZIKV感染的HTR 8细胞。这些DEG中的几个在与可能导致胎儿损伤的每种细胞类型的特征相关的不同生物过程中富集。两种细胞类型在ZIKV感染后均表现出常见干扰素、炎性细胞因子和趋化因子产生的活化。此外，肿瘤坏死因子-α（TNF-α）的中和促进滋养层细胞和胶质母细胞瘤星形细胞瘤细胞中的ZIKV感染。总之，我们鉴定了与ZIKV发病机制相关的多种DEG。
ZIKV has a different infection pattern for placental trophoblasts and human brain cells. ZIKV infection induced interferons, inflammatory cytokines, and chemokine production. TNF-α neutralisation could promote ZIKV infection between HTR8 and U251 cells. The mechanism by which Zika virus (ZIKV) causes severe birth defects in pregnant women remains unclear. Cell tropisms in placenta and brain play a crucial role in ZIKV pathogenesis, leading to congenital Zika syndrome (CZS). To identify the host factors involved in ZIKV infection, we compared the transcriptional profiles of ZIKV-infected human first-trimester placental trophoblast cells HTR8/SVneo and a human glioblastoma astrocytoma cell line U251. Our results demonstrated that ZIKV exhibited lower rates of mRNA replication and protein expression in HTR8 than in U251 cells, while showing a higher release of infectious viral particles. However, a greater number of differentially expressed genes (DEGs) were found in ZIKV-infected U251 cells than in ZIKV-infected HTR8 cells. Several of these DEGs were enriched in distinct biological processes related to the characteristics of each cell type that may contribute to foetal damage. Both cell types exhibited activation of common interferons, inflammatory cytokines, and chemokine production upon ZIKV infection. Moreover, the neutralization of tumour necrosis factor-alpha (TNF-α) promoted ZIKV infection in both trophoblasts and glioblastoma astrocytoma cells. Overall, we identified multiple DEGs associated with ZIKV pathogenesis.
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影响因子：
4.6
作者：
Kumar A;Jovel J;Lopez-Orozco J;Limonta D;Airo AM;Hou S;Stryapunina I;Fibke C;Moore RB;Hobman TC
通讯作者：
Hobman TC
影响因子：
16.6
作者：
Caires-Júnior LC;Goulart E;Melo US;Araujo BHS;Alvizi L;Soares-Schanoski A;de Oliveira DF;Kobayashi GS;Griesi-Oliveira K;Musso CM;Amaral MS;daSilva LF;Astray RM;Suárez-Patiño SF;Ventini DC;Gomes da Silva S;Yamamoto GL;Ezquina S;Naslavsky MS;Telles-Silva KA;Weinmann K;van der Linden V;van der Linden H;de Oliveira JRM;Arrais NMR;Melo A;Figueiredo T;Santos S;Meira JGC;Passos SD;de Almeida RP;Bispo AJB;Cavalheiro EA;Kalil J;Cunha-Neto E;Nakaya H;Andreata-Santos R;de Souza Ferreira LC;Verjovski-Almeida S;Ho PL;Passos-Bueno MR;Zatz M
通讯作者：
Zatz M
影响因子：
16.6
作者：
Hirsch, Alec J.;Roberts, Victoria H. J.;Streblow, Daniel N.
通讯作者：
Streblow, Daniel N.
影响因子：
158.5
作者：
Driggers, R. W.;Ho, C. -Y.;Vapalahti, O.
通讯作者：
Vapalahti, O.
影响因子：
6
作者：
de Sousa, Jorge R.;Azevedo, Raimunda S. S.;Vasconcelos, Pedro F. C.
通讯作者：
Vasconcelos, Pedro F. C.