Genome-wide identification and analysis of MAPK and MAPKK gene families in Brachypodium distachyon.
Genome-wide identification and analysis of MAPK and MAPKK gene families in Brachypodium distachyon.
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DOI：
10.1371/journal.pone.0046744
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
He G
中科院分区：
文献类型：
--
作者：
Chen L;Hu W;Tan S;Wang M;Ma Z;Zhou S;Deng X;Zhang Y;Huang C;Yang G;He G
MAPK级联反应是一种普遍存在的信号转导模块，在植物生长发育及对各种生物和非生物胁迫的应答中起着重要作用。虽然MAPKs和MAPKKs已在包括拟南芥、水稻和白杨在内的几种植物物种中进行了系统的研究，但尚未在新兴的单子叶模式植物二穗短柄草中进行系统的分析。在本研究中，从B中共鉴定出16个MAPK基因和12个MAPKK基因。二穗子对基因组进化的分析表明，串联重复和片段重复都对MAPK和MAPKK家族的扩展有重要贡献。亚家族内的进化关系得到了外显子-内含子组织和保守蛋白质基序结构的支持。B.二穗草和水稻、拟南芥等两种植物中只有一个B的同源物。在拟南芥的同线区发现了13个二穗型MAPKs，而B的同源物有13个。在水稻中发现了二穗草MAPKs和MAPKK，这与三个物种的物种形成过程一致。此外，在B中还发现了两个家族间的几个互作蛋白对。通过酵母双杂交试验发现，它们在拟南芥和其他植物中的一对直向同源物没有发现相互作用。最后，对B中密切相关的家族成员进行了表达研究。二穗草、拟南芥和水稻的研究表明，即使是最近复制的代表也可能履行不同的功能，参与不同的信号通路。综上所述，我们的数据将提供一个在B的MAPK和MAPKK基因家族的进化和功能的研究基础。二穗草和其他植物物种，以解开他们的生物作用。
MAPK cascades are universal signal transduction modules and play important roles in plant growth, development and in response to a variety of biotic and abiotic stresses. Although MAPKs and MAPKKs have been systematically investigated in several plant species including Arabidopsis, rice and poplar, no systematic analysis has been conducted in the emerging monocot model plant Brachypodium distachyon. In the present study, a total of 16 MAPK genes and 12 MAPKK genes were identified from B. distachyon. An analysis of the genomic evolution showed that both tandem and segment duplications contributed significantly to the expansion of MAPK and MAPKK families. Evolutionary relationships within subfamilies were supported by exon-intron organizations and the architectures of conserved protein motifs. Synteny analysis between B. distachyon and the other two plant species of rice and Arabidopsis showed that only one homolog of B. distachyon MAPKs was found in the corresponding syntenic blocks of Arabidopsis, while 13 homologs of B. distachyon MAPKs and MAPKKs were found in that of rice, which was consistent with the speciation process of the three species. In addition, several interactive protein pairs between the two families in B. distachyon were found through yeast two hybrid assay, whereas their orthologs of a pair in Arabidopsis and other plant species were not found to interact with each other. Finally, expression studies of closely related family members among B. distachyon, Arabidopsis and rice showed that even recently duplicated representatives may fulfill different functions and be involved in different signal pathways. Taken together, our data would provide a foundation for evolutionary and functional characterization of MAPK and MAPKK gene families in B. distachyon and other plant species to unravel their biological roles.
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