WRKY Transcription Factors Associated With NPR1-Mediated Acquired Resistance in Barley Are Potential Resources to Improve Wheat Resistance to Puccinia triticina.
WRKY Transcription Factors Associated With NPR1-Mediated Acquired Resistance in Barley Are Potential Resources to Improve Wheat Resistance to Puccinia triticina.
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DOI：
10.3389/fpls.2018.01486
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发表时间：
2018
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Gao J;Bi W;Li H;Wu J;Yu X;Liu D;Wang X
拟南芥的系统获得性抗性（SAR）是在最初的病原性感染之后建立的，或者是通过水杨酸或其功能类似物（SA/INA/BTH）处理直接诱导的。 NPR1 蛋白和 WRKY 转录因子被认为是 SAR 的主要调节因子。我们之前的研究表明，小麦（Triticum aestivum L.）和大麦（Hordeum vulgare L.）中的NPR1同源物在丁香假单胞菌引发的获得性抗性（AR）过程中调节编码发病机制相关（PR）蛋白的基因的表达。番茄DC3000。在本次检测中，还发现丁香假单胞菌DC3000诱导的AR能够有效提高小麦对小麦锈菌（Pt）的抗性。然而，由于基因组更加复杂，与小麦和大麦中这种 SAR 样反应相关的基因在很大程度上是未知的，并且没有报道称特定的 WRKY 参与了这一生物过程。在我们随后的分析中，过表达小麦 wNPR1 (wNPR1-OE) 的大麦转基因品系显示出对稻瘟病菌隔离株 Guy11 的抗性增强，而在敲除大麦 HvNPR1 (HvNPR1-Kd) 的大麦转基因品系中，Guy11 的 AR 受到抑制。我们进行了 RNA 测序，以揭示 AR 期间这些转基因品系和野生型大麦植株之间差异表达的基因。几个 PR 和 BTH 诱导 (BCI) 基因被指定为 NPR1 的下游基因。 AR 事件期间少数 WRKY 的表达与 NPR1 的表达显着相关。通过农杆菌介导的渗透，小麦叶片中三个WRKY基因（包括HvWRKY6、HvWRKY40和HvWRKY70）的瞬时表达增强了对Pt的抗性。总之，起草了大麦中与 NPR1 介导的 AR 相关的基因谱，当前研究中发现的 WRKY 显示了提高小麦对 Pt 抗性的巨大潜力。
Systemic acquired resistance (SAR) in Arabidopsis is established beyond the initial pathogenic infection or is directly induced by treatment with salicylic acid or its functional analogs (SA/INA/BTH). NPR1 protein and WRKY transcription factors are considered the master regulators of SAR. Our previous study showed that NPR1 homologs in wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) regulated the expression of genes encoding pathogenesis-related (PR) proteins during acquired resistance (AR) triggered by Pseudomonas syringae pv. tomato DC3000. In the present examination, AR induced by P. syringae DC3000 was also found to effectively improve wheat resistance to Puccinia triticina (Pt). However, with more complex genomes, genes associated with this SAR-like response in wheat and barley are largely unknown and no specific WRKYs has been reported to be involved in this biological process. In our subsequent analysis, barley transgenic line overexpressing wheat wNPR1 (wNPR1-OE) showed enhanced resistance to Magnaporthe oryzae isolate Guy11, whereas AR to Guy11 was suppressed in a barley transgenic line with knocked-down barley HvNPR1 (HvNPR1-Kd). We performed RNA-seq to reveal the genes that were differentially expressed among these transgenic lines and the wild-type barley plants during the AR. Several PR and BTH-induced (BCI) genes were designated as downstream genes of NPR1. The expression of few WRKYs was significantly associated with NPR1 expression during the AR events. The transient expression of three WRKY genes, including HvWRKY6, HvWRKY40, and HvWRKY70, in wheat leaves by Agrobacterium-mediated infiltration enhanced the resistance to Pt. In conclusion, a profile of genes associated with NPR1-mediated AR in barley was drafted and WRKYs discovered in the current study showed a substantial potential for improving wheat resistance to Pt.
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影响因子：
5.3
作者：
Nakayama A;Fukushima S;Goto S;Matsushita A;Shimono M;Sugano S;Jiang CJ;Akagi A;Yamazaki M;Inoue H;Takatsuji H
通讯作者：
Takatsuji H
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN
影响因子：
3.5
作者：
Molina, A;Görlach, J;Ryals, J
通讯作者：
Ryals, J
影响因子：
3.5
作者：
Shah, J;Tsui, F;Klessig, DF
通讯作者：
Klessig, DF
DOI：
10.1073/pnas.92.14.6602
发表时间：
1995-07-03
影响因子：
11.1
作者：
DELANEY, TP;FRIEDRICH, L;RYALS, JA
通讯作者：
RYALS, JA